| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E |
| Entrez GeneID | 6490 |
| clone | 5C6G8 |
| WB Predicted band size | 70.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PMEL (AA: 25-192) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于Plk1 (Phospho-Thr210)抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**：*"Autoactivation of Polo-like Kinase 1 (Plk1) by Phosphorylation of Thr210"*
**作者**：Jung JH et al.
**摘要**：该研究阐明了Plk1通过Thr210位点的自磷酸化激活机制。作者利用Phospho-Thr210特异性抗体，通过免疫印迹和免疫荧光验证了Plk1在细胞有丝分裂中的激活动态，并证明该位点的磷酸化是其激酶活性的关键调节步骤。
2. **文献名称**：*"Phosphorylation of Thr210 in the Activation Loop of Plk1 is Required for Cell Cycle Progression"*
**作者**：Lenart P et al.
**摘要**：研究通过Phospho-Thr210抗体发现，Plk1的Thr210磷酸化是细胞周期G2/M期转换的必要条件。抗体被用于检测HeLa细胞中Plk1的激活状态，并证明其磷酸化水平与有丝分裂进程密切相关。
3. **文献名称**：*"DNA Damage-Induced Inhibition of Plk1 Activity Involves Dephosphorylation of Thr210"*
**作者**：Smits VAJ et al.
**摘要**：文章揭示了DNA损伤后Plk1活性下调的机制。通过Phospho-Thr210抗体，作者发现DNA损伤信号(如电离辐射)导致Thr210去磷酸化，从而抑制Plk1功能，表明该位点磷酸化状态与基因组稳定性相关。
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这些文献均通过Phospho-Thr210抗体研究Plk1的功能调控，涵盖激酶激活机制、细胞周期调控及DNA损伤应答等方向。
The Polo-like kinase 1 (Plk1) is a serine/threonine kinase critical for regulating cell cycle progression, particularly during mitosis. Its activity is tightly controlled by phosphorylation, and phosphorylation at threonine 210 (Thr210) in the T-loop of its catalytic domain is essential for full kinase activation. This post-translational modification stabilizes the active conformation of Plk1. enabling its roles in centrosome maturation, spindle assembly, chromosome segregation, and mitotic exit.
Plk1 phosphorylates numerous substrates involved in mitosis, and its dysregulation is linked to genomic instability and cancer. Overexpression of Plk1 is observed in various malignancies, making it a potential therapeutic target. The Phospho-Thr210 antibody specifically detects Plk1 when phosphorylated at Thr210. serving as a key tool to study Plk1 activation dynamics during the cell cycle. Researchers use this antibody in techniques like Western blotting, immunofluorescence, and flow cytometry to assess Plk1 activity in synchronized cells, mitotic arrest models, or cancer samples.
Phospho-Thr210 levels peak during G2/M phase and decline upon mitotic exit, correlating with Plk1’s functional window. Studies employing this antibody have elucidated Plk1’s role in checkpoint recovery, DNA damage response, and its crosstalk with other mitotic kinases. Its specificity also aids in evaluating Plk1 inhibitors in preclinical cancer research, providing insights into drug efficacy and mechanism of action.
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