Rabbit Polyclonal PDGFR-β(Phospho-Tyr1021) Antibody

The PDGFR-β (Phospho-Tyr1021) antibody is designed to detect the activated form of platelet-derived growth factor receptor beta (PDGFR-β) when phosphorylated at tyrosine residue 1021. PDGFR-β, a receptor tyrosine kinase, plays a critical role in regulating cell proliferation, migration, and survival. Upon binding to PDGF ligands (e.g., PDGF-BB), the receptor dimerizes, leading to autophosphorylation of specific tyrosine residues, including Tyr1021. within its kinase domain. This phosphorylation event triggers downstream signaling cascades, such as the Ras/MAPK and PI3K/Akt pathways, which drive cellular responses in development, tissue repair, and disease. Dysregulation of PDGFR-β signaling is implicated in cancers, fibrosis, and cardiovascular disorders. The Phospho-Tyr1021 antibody specifically recognizes the activated receptor, enabling researchers to study PDGFR-β activation status in experimental models or clinical samples. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess receptor activation dynamics, evaluate therapeutic inhibitors, or correlate signaling activity with pathological conditions. Specificity is typically validated using phosphorylation-blocking peptides or cells with PDGFR-β knockout. Proper sample preparation, including phosphatase inhibition, is essential to preserve phosphorylation signals.