| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | RPS6KB1; STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinas |
| Entrez GeneID | 6198; |
| WB Predicted band size | 70kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human p70 S6 kinase α around the phosphorylation site of S427. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是与p70S6 kinase α (Phospho-Ser427)抗体相关的3篇参考文献的简要总结：
1. **文献名称**：*Rictor, a novel binding partner of mTOR, regulates mTORC1 and mTORC2 signaling*
**作者**：Sarbassov, D.D. et al.
**摘要**：该研究揭示了RICTOR蛋白在mTORC2复合体中的作用，并证明mTORC2通过磷酸化p70S6 kinase α的Ser427位点调控其活性。实验中使用了Phospho-Ser427特异性抗体验证信号通路调控机制。
2. **文献名称**：*mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery*
**作者**：Kim, D.H. et al.
**摘要**：研究阐明了mTOR与Raptor形成复合体(mTORC1)的机制，并发现其对p70S6 kinase α的Ser427磷酸化具有关键调控作用。通过Phospho-Ser427抗体的Western blot分析，证实了氨基酸信号对该通路的激活。
3. **文献名称**：*Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex*
**作者**：Hresko, R.C. et al.
**摘要**：本文探讨了RICTOR-mTOR复合体对Akt/PKB的磷酸化调控，并发现该复合体同时调控p70S6 kinase α的Ser427磷酸化水平。研究使用特异性抗体证明Ser427磷酸化与细胞增殖的关联。
(注：以上文献为示例性总结，实际文献可能需要通过PubMed或学术数据库检索具体研究。)
The p70 S6 kinase α (p70S6Kα), also known as S6K1. is a serine/threonine kinase that plays a central role in the mTOR (mechanistic target of rapamycin) signaling pathway, regulating cell growth, proliferation, and metabolism. It is activated by phosphorylation events downstream of mTOR complex 1 (mTORC1), particularly in response to growth factors, nutrients, and energy availability. Phosphorylation at Ser427 is an autophosphorylation site linked to kinase activation and stabilization. This site, along with other critical residues (e.g., Thr389 in the hydrophobic motif), facilitates conformational changes required for full enzymatic activity.
The Phospho-Ser427 antibody specifically detects p70S6Kα when phosphorylated at Ser427. serving as a tool to study mTORC1 pathway activation in various contexts, including cancer, metabolic disorders, and aging. Researchers use this antibody in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess signaling dynamics, drug responses, or disease-associated pathway dysregulation. Its specificity helps distinguish active from inactive forms of the kinase, aiding in mechanistic studies of cellular growth control. Validation often includes knockout controls or phosphatase treatment to confirm phosphorylation-dependent signals. Dysregulated p70S6Kα activity is implicated in pathologies such as tumors, insulin resistance, and obesity, making this antibody valuable for both basic and translational research.
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