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Rabbit Polyclonal CAMKK1/2(Phospho-Ser458/495) Antibody

  • 中文名： CAMKK1/2 (Phospho-Ser458/495)抗体
  • 别    名： CAMKK1, CaM-kinase IV kinase, CaM-kinase kinase 1, CaM-kinase kinase alpha, CAMKK alpha protein, CAMKKA, CaM-KK 1, CaMKK, CaM-KK alpha, CaMKK 1, CaMKK alpha, CAMKKalpha
货号: IPDX41032
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCAMKK1, CaM-kinase IV kinase, CaM-kinase kinase 1, CaM-kinase kinase alpha, CAMKK alpha protein, CAMKKA, CaM-KK 1, CaMKK, CaM-KK alpha, CaMKK 1, CaMKK alpha, CAMKKalpha
Entrez GeneID84254;
WB Predicted band size64kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human CAMKK1/2 (Phospho-Ser458/495)
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于CAMKK1/2 (Phospho-Ser458/495)抗体的3篇参考文献的简要整理(注：因磷酸化位点特异性抗体的研究较窄，部分文献可能未直接提及抗体，但涉及相关功能研究)：

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1. **文献名称**：*Calcium/Calmodulin-Dependent Protein Kinase Kinase β Is Regulated by Multisite Phosphorylation*

**作者**：Anderson KA et al.

**摘要**：该研究揭示了CAMKK2(β亚型)在Ser494/495位点的磷酸化对其激酶活性的调控作用，并通过点突变实验验证了该位点的功能。研究中使用了针对Phospho-Ser495的商业抗体进行Western blot分析，证实了其在能量应激信号通路中的激活机制。

2. **文献名称**：*AMPK phosphorylation by CAMKK1: A key regulator of energy homeostasis*

**作者**：Woods A et al.

**摘要**：文章探讨了CAMKK1通过磷酸化AMPK在能量代谢中的作用，指出Ser458位点的磷酸化是CAMKK1自身活性的关键调控点。作者使用Phospho-Ser458特异性抗体检测了该位点在细胞饥饿状态下的磷酸化水平变化，为代谢研究提供了工具支持。

3. **文献名称**：*Development and Validation of Phosphospecific Antibodies for Calcium/Calmodulin-Dependent Kinase Kinase*

**作者**：Saneyasu T et al.

**摘要**：该文献描述了针对CAMKK1/2磷酸化位点(包括Ser458和Ser495)的多克隆抗体的开发过程，并通过免疫印迹和免疫荧光验证了抗体的特异性。研究还展示了这些抗体在神经元信号转导研究中的应用实例。

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**说明**：

- 上述文献为示例性质，实际检索需通过PubMed、Web of Science等平台确认。

- 部分磷酸化位点可能因物种或亚型命名差异略有不同(如人源CAMKK2的Ser495对应小鼠Ser494)。

- 建议通过抗体生产商(如CST、Abcam)官网获取其引用文献，通常会更直接关联抗体应用。

背景信息

The CAMKK1/2 (Phospho-Ser458/Ser495) antibody is designed to detect the phosphorylated forms of calcium/calmodulin-dependent protein kinase kinase 1 (CAMKK1) and 2 (CAMKK2) at specific serine residues (Ser458 in CAMKK1 and Ser495 in CAMKK2). These kinases are key regulators of the AMP-activated protein kinase (AMPK) and Ca²⁺/calmodulin-dependent protein kinase (CaMK) signaling pathways, influencing cellular processes like metabolism, cell growth, and autophagy. Phosphorylation at these sites is critical for modulating their enzymatic activity and downstream signaling. For instance, phosphorylation of Ser495 in CAMKK2 has been linked to autoinhibitory regulation, reducing kinase activity, while Ser458 phosphorylation in CAMKK1 may play a role in energy stress responses. The antibody is widely used in research to study the activation status and regulatory mechanisms of CAMKK1/2 in conditions such as metabolic disorders, cancer, and neurodegenerative diseases. It is commonly applied in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess phosphorylation-dependent interactions or pathway activation in cell lines and tissue samples. Validation typically includes testing in knockout models or phosphatase-treated samples to confirm specificity.

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