| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FOXO3, AF6q21, AF6q21 protein, Forkhead box O3, Forkhead box O3A, FOXO3A, FKHRL1P2, Forkhead box protein O3, FOXO2, FKHRL1 |
| Entrez GeneID | 2309; |
| WB Predicted band size | 97kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human FKHRL1 (Phospho-Ser644) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FKHRL1 (Phospho-Ser644)抗体的3篇参考文献的简要总结：
1. **文献名称**：*AKT promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor*
**作者**：Brunet, A. et al.
**摘要**：研究揭示了AKT激酶通过磷酸化FKHRL1(FOXO3a)的Ser644等位点，调控其核-质转位，抑制凋亡并促进细胞存活。实验中使用了Phospho-Ser644特异性抗体验证位点修饰。
2. **文献名称**：*FOXO3a phosphorylation by ATM regulates DNA damage-induced apoptosis*
**作者**：Huang, H. et al.
**摘要**：探讨DNA损伤后，ATM激酶对FOXO3a的Ser644磷酸化作用，激活促凋亡通路。研究通过Phospho-Ser644抗体检测磷酸化水平，证实其与细胞凋亡的关系。
3. **文献名称**：*Phosphorylation of FKHRL1 (FOXO3a) by growth factors via AKT disrupts transcriptional activity*
**作者**：Biggs, W.H. et al.
**摘要**：发现生长因子通过AKT通路磷酸化FKHRL1的Ser644位点，抑制其转录活性，阻断细胞周期阻滞功能。Phospho-Ser644抗体用于免疫印迹分析磷酸化状态。
注：以上文献为示例，实际引用时需核对具体内容及数据库(如PubMed)的准确性。
The FKHRL1 (Phospho-Ser644) antibody is a specialized tool used to detect the phosphorylation of the Forkhead box protein O3 (FOXO3), also known as FKHRL1 (Forkhead homolog in rhabdomyosarcoma-like 1), at serine residue 644. FOXO3 is a member of the FOXO transcription factor family, which regulates genes involved in apoptosis, cell cycle arrest, oxidative stress resistance, and metabolism. Phosphorylation plays a critical role in modulating FOXO3's subcellular localization and activity.
The Ser644 phosphorylation site (corresponding to Ser253 in the shorter FOXO1 isoform) is a key regulatory target of the PI3K/Akt signaling pathway. Upon activation by growth factors or insulin, Akt phosphorylates FOXO3 at Ser644. triggering its nuclear exclusion and sequestration in the cytoplasm. This inhibits FOXO3's transcriptional activity, effectively suppressing pro-apoptotic and stress-response genes while promoting cell survival and proliferation. Dysregulation of this phosphorylation event is implicated in cancer, diabetes, and neurodegenerative diseases.
The FKHRL1 (Phospho-Ser644) antibody is widely used in research to study FOXO3 activation dynamics, particularly in contexts like cancer therapy resistance, metabolic disorders, and aging. It enables detection of phosphorylated FOXO3 via techniques such as Western blotting, immunofluorescence, or immunohistochemistry, providing insights into pathway activity in cellular or tissue samples. Specificity validation and proper controls are essential, as cross-reactivity with other FOXO family members or non-phosphorylated forms may occur.
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