Rabbit Polyclonal VAMP4(Phospho-Ser30) Antibody

The VAMP4 (Phospho-Ser30) antibody is designed to detect the phosphorylation of vesicle-associated membrane protein 4 (VAMP4) at serine residue 30. a post-translational modification critical for regulating its function in intracellular membrane trafficking. VAMP4. a member of the SNARE protein family, facilitates vesicle docking and fusion between organelles, particularly in the trans-Golgi network (TGN) and endosomal compartments. Its N-terminal domain contains regulatory motifs, including a phosphorylation site at Ser30. which modulates interactions with adaptor proteins like AP-1 or GGAs during clathrin-coated vesicle formation. Phosphorylation at Ser30 is thought to influence VAMP4’s localization, stability, or binding partners, thereby affecting cargo sorting and vesicular transport. This antibody is widely used in studies exploring membrane trafficking dynamics, neurosecretory pathways, or diseases linked to SNARE dysfunction, such as neurological disorders or secretion defects. Researchers employ it in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess phosphorylation-dependent VAMP4 behavior under various physiological or pathological conditions. Validation often includes knockout controls or phosphatase treatment to confirm specificity. Understanding VAMP4 phosphorylation provides insights into cellular trafficking mechanisms and potential therapeutic targets.