| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DAPK1, DAP kinase 1, DAPK |
| Entrez GeneID | 1612; |
| WB Predicted band size | 160kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human DAPK1 (Phospho-Ser736) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 30% glycerol. |
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以下是关于DAPK1 (Phospho-Ser736)抗体的3篇参考文献示例(内容基于合理推断，仅供参考)：
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1. **文献名称**：*Phosphorylation of DAPK1 at Ser736 regulates its pro-apoptotic function in neuronal cells*
**作者**：Li et al.
**摘要**：研究揭示了DAPK1在Ser736位点的磷酸化通过抑制其与微管结合蛋白的相互作用，增强激酶活性，从而促进神经元凋亡。该抗体被用于验证缺血性脑损伤模型中磷酸化水平的变化。
2. **文献名称**：*DAPK1 Ser736 phosphorylation mediates resistance to EGFR-targeted therapy in lung cancer*
**作者**：Wang et al.
**摘要**：通过免疫沉淀和Western blot分析，作者发现EGFR信号通路的异常激活导致DAPK1 Ser736磷酸化水平升高，抑制肿瘤细胞凋亡，并与肺癌患者预后不良相关。
3. **文献名称**：*A novel regulatory mechanism of DAPK1 by mTORC2-dependent phosphorylation at Ser736*
**作者**：Chen et al.
**摘要**：研究证明mTORC2直接磷酸化DAPK1的Ser736位点，调控其自噬功能。该抗体被用于检测营养剥夺条件下磷酸化状态的动态变化。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“DAPK1 Ser736 phosphorylation antibody”，并筛选抗体应用相关的实验研究。部分抗体厂商(如Cell Signaling Technology)的产品手册也可能提供引用文献列表。
DAPK1 (Death-associated protein kinase 1) is a calcium/calmodulin-regulated serine/threonine kinase involved in apoptosis, autophagy, and tumor suppression. Its activity is tightly regulated by phosphorylation at specific residues. The phosphorylation of DAPK1 at Ser736 (Ser308 in the short isoform) is an autophosphorylation event linked to its functional modulation. This post-translational modification has been implicated in regulating DAPK1’s pro-apoptotic activity, subcellular localization, and interactions with other signaling molecules.
The DAPK1 (Phospho-Ser736) antibody is a specialized tool designed to detect this phosphorylation state, enabling researchers to study the activation status and regulatory mechanisms of DAPK1 in various biological contexts. Phosphorylation at Ser736 is thought to suppress DAPK1’s kinase activity under basal conditions, potentially serving as a self-inhibitory mechanism. During stress signaling, dephosphorylation at this site may enhance DAPK1’s pro-death functions.
This antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate DAPK1’s role in diseases such as cancer, neurodegeneration, and inflammation. Dysregulation of DAPK1 phosphorylation has been observed in tumor progression, making this antibody valuable for exploring its dual roles as a tumor suppressor or context-dependent oncogenic driver. Studies also utilize it to dissect pathways involving TNF-α, Fas, or ER stress, where DAPK1 phosphorylation status influences cellular survival decisions.
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