| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP3K2, MAP/ERK kinase kinase 2, MAPK/ERK kinase kinase 2, MAPKKK2, MEK kinase 2, MEKK 2, MEKK2, MEKK2B |
| Entrez GeneID | 10746; |
| WB Predicted band size | 70kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human MAP3K2 (Phospho-Ser520) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及MAP3K2 (Phospho-Ser520)抗体的参考文献示例(注：内容为示例性概括，实际文献需根据具体数据库检索)：
1. **文献名称**：*"MAP3K2 phosphorylation at Ser520 links RAS signaling to sustained ERK activation in tumorigenesis"*
**作者**：Smith J et al.
**摘要**：本研究揭示MAP3K2在Ser520位点的磷酸化是RAS信号下游ERK持续激活的关键步骤，通过特异性抗体验证了该位点在多种癌细胞中的磷酸化水平与肿瘤增殖的相关性。
2. **文献名称**：*"Phosphorylation-dependent regulation of MAP3K2/MEKK2 in inflammatory signaling pathways"*
**作者**：Chen L et al.
**摘要**：利用Phospho-Ser520抗体，作者发现炎症因子TNF-α通过激活MAP3K2的Ser520磷酸化，调控JNK/p38通路，促进巨噬细胞炎症反应。
3. **文献名称**：*"Functional analysis of MAP3K2 Ser520 phosphorylation in cardiac hypertrophy"*
**作者**：Wang Y et al.
**摘要**：通过Western blot和免疫荧光使用Phospho-Ser520抗体，证实压力超负荷诱导的心肌细胞肥大中MAP3K2磷酸化水平升高，提示其参与病理性心脏重塑。
建议通过PubMed或抗体供应商(如CST、Abcam)官网的引用列表获取真实文献信息。
The MAP3K2 (Phospho-Ser520) antibody is designed to detect the mitogen-activated protein kinase kinase kinase 2 (MAP3K2) when phosphorylated at serine 520. a post-translational modification critical for regulating its enzymatic activity. MAP3K2. also known as MEKK2. is a serine/threonine kinase that plays a key role in signaling cascades, including the MAPK/ERK pathway, which influences cell proliferation, differentiation, and stress responses. Phosphorylation at Ser520. located within the kinase activation loop, is associated with enhanced kinase activity and downstream signaling. This modification often occurs in response to extracellular stimuli, such as growth factors or cytokines, and is tightly regulated by upstream kinases or autophosphorylation.
The antibody is typically a polyclonal or monoclonal reagent validated for applications like Western blotting, immunoprecipitation, or immunofluorescence to study endogenous phospho-MAP3K2 levels in cell lysates or tissues. Researchers use it to explore MAP3K2's role in physiological processes, including immune regulation, angiogenesis, and cancer progression. Dysregulation of MAP3K2 signaling has been implicated in diseases such as inflammatory disorders and RAS-driven tumors. Detecting Ser520 phosphorylation helps assess MAP3K2 activation status in experimental models, aiding mechanistic studies of pathway crosstalk or therapeutic targeting. Proper controls, like phosphorylation-blocking peptides or kinase-inactive mutants, are recommended to confirm specificity.
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