Rabbit Polyclonal Calpain2(Phospho-Ser50) Antibody

Calpain 2 (Phospho-Ser50) antibody is a specialized tool used to detect the phosphorylated form of Calpain-2 at serine residue 50 (Ser50). Calpain-2. a calcium-dependent cysteine protease, belongs to the calpain family, which plays critical roles in cellular processes such as cell migration, apoptosis, and signal transduction. Unlike its isoform Calpain-1. Calpain-2 requires higher calcium concentrations for activation and often functions as a heterodimer composed of a large catalytic subunit (encoded by *CAPN2*) and a small regulatory subunit (CAPNS1). Phosphorylation at Ser50. located in the regulatory domain of Calpain-2. is a key post-translational modification that modulates its enzymatic activity, subcellular localization, and interaction with substrates. This phosphorylation event, mediated by kinases like ERK1/2. enhances Calpain-2 activation under physiological or pathological conditions, including cancer progression, neurodegeneration, and ischemia-reperfusion injury. The Calpain 2 (Phospho-Ser50) antibody is widely utilized in research to investigate the regulatory mechanisms of Calpain-2 activity in disease models, particularly in studies involving tissue remodeling, inflammation, or cell death pathways. Its specificity for the phosphorylated epitope allows precise detection of activated Calpain-2 in Western blotting, immunohistochemistry, or immunofluorescence, aiding in the exploration of its role in cellular stress responses and therapeutic targeting.