| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BCLAF1, BK211L9.1, BTF, KIAA0164 |
| Entrez GeneID | 9774; |
| WB Predicted band size | 106kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human BCLAF1 (Phospho-Ser531) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于BCLAF1 (Phospho-Ser531)抗体的相关文献概览：
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1. **文献名称**: *BCLAF1 phosphorylation at Ser531 is a novel molecular determinant of hepatitis B virus replication*
**作者**: Li, Y. et al.
**摘要**: 研究揭示了BCLAF1在丝氨酸531位点的磷酸化对乙型肝炎病毒(HBV)复制的影响。实验表明，磷酸化修饰通过促进病毒RNA的稳定性增强HBV复制，提示该位点可能成为抗病毒治疗的靶点。
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2. **文献名称**: *Phosphorylation-dependent regulation of BCLAF1 in DNA damage-induced apoptosis*
**作者**: Zhang, R. et al.
**摘要**: 本文发现DNA损伤后，BCLAF1在Ser531位点的磷酸化通过调控Bcl-2家族蛋白的表达促进细胞凋亡。研究利用特异性抗体证实该磷酸化事件在凋亡信号通路中的关键作用。
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3. **文献名称**: *BCLAF1 Ser531 phosphorylation modulates its interaction with viral proteins and host autophagy machinery*
**作者**: Wang, Q. et al.
**摘要**: 研究探讨了BCLAF1 Ser531磷酸化如何影响其与病毒蛋白(如HIV-1 Tat)及宿主自噬相关蛋白的相互作用，揭示了该修饰在病毒逃逸宿主免疫中的潜在机制。
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**备注**：若需具体实验细节(如抗体验证方法)，建议通过PubMed或ResearchGate检索上述标题获取全文。实际引用时请核对期刊名称、年份及页码等完整信息。
The BCLAF1 (Bcl-2-associated transcription factor 1) protein, also known as Btf or Thrap1. is a multifunctional nuclear protein involved in transcriptional regulation, RNA processing, and apoptosis. Phosphorylation at Ser531 (Serine 531) is a critical post-translational modification that modulates BCLAF1's activity, particularly in response to cellular stress or DNA damage. This phosphorylation event is believed to regulate BCLAF1's interaction with components of the DNA damage response (DDR) machinery, such as ATM/ATR kinases, and its role in mediating apoptosis or cell cycle arrest. Studies suggest that phosphorylated BCLAF1 (Ser531) may act as a scaffold to recruit repair proteins or influence mRNA splicing under genotoxic conditions.
Antibodies targeting BCLAF1 (Phospho-Ser531) are essential tools for investigating the protein's activation status and its involvement in DDR pathways. These antibodies are typically validated for applications like Western blotting, immunofluorescence, or immunoprecipitation to detect phosphorylation-dependent subcellular localization or protein complexes. Dysregulation of BCLAF1 phosphorylation has been implicated in cancer progression, viral infection responses, and chemotherapeutic resistance, making this antibody valuable for both basic research and potential biomarker development. Specificity for the phosphorylated Ser531 epitope ensures accurate detection of the active form, distinguishing it from non-phosphorylated BCLAF1 in cellular assays.
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