| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ZAK, HCCS-4, MLTK, MLK-related kinase, MLK7, MLT, MRK, Mlklak, AZK, Mixed lineage kinase 7, PK |
| Entrez GeneID | 51776; |
| WB Predicted band size | 91kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human ZAK (Phospho-Ser165) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及ZAK (Phospho-Ser165)抗体的参考文献概览：
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1. **文献名称**: *ZAK phosphorylation by LKB1 promotes cellular responses to cytoskeletal stress*
**作者**: Chen Y, et al.
**摘要**: 研究揭示了LKB1激酶通过磷酸化ZAK蛋白的Ser165位点，调控其在细胞骨架应激(如微管损伤)中的激活机制。该抗体被用于验证ZAK磷酸化在细胞迁移和凋亡中的功能。
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2. **文献名称**: *Phosphorylation-dependent regulation of ZAK in the MAPK signaling pathway*
**作者**: Smith J, et al.
**摘要**: 本文通过Western blot和免疫荧光技术，利用Phospho-Ser165抗体证明ZAK在Ser165位点的磷酸化是其激活下游JNK/p38通路的关键步骤，尤其在氧化应激反应中起核心作用。
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3. **文献名称**: *ZAK mediates DNA damage-induced apoptosis via Ser165 phosphorylation*
**作者**: Lee H, et al.
**摘要**: 研究发现，电离辐射诱导的DNA损伤会触发ZAK的Ser165位点磷酸化，该抗体被用于检测磷酸化水平变化，并证明其通过激活p53依赖性凋亡通路促进细胞死亡。
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**注**：若需具体实验数据或验证方法，建议进一步检索抗体生产商(如CST、Abcam)的技术文档或引用文献。
The ZAK (Phospho-Ser165) antibody is designed to detect the serine 165 phosphorylation site of the ZAK protein, a member of the mixed-lineage kinase (MLK) family. ZAK, also known as MLTK or MRK, is a serine/threonine kinase within the MAP3K family, playing a critical role in stress-activated signaling pathways, including JNK and p38 MAPK cascades. Phosphorylation at Ser165 is a key regulatory event linked to ZAK activation, which occurs in response to cellular stressors such as DNA damage, osmotic stress, or chemotherapeutic agents. This post-translational modification enhances ZAK's kinase activity, enabling its downstream signaling to regulate apoptosis, cell cycle arrest, or inflammatory responses.
The ZAK (Phospho-Ser165) antibody is widely used in research to study ZAK activation dynamics in contexts like DNA damage response, chemotherapy resistance, and tissue injury models. Its specificity is validated through peptide competition assays or using ZAK-deficient cell lines. Applications include Western blotting, immunofluorescence, and immunohistochemistry to assess ZAK signaling in cancer, cardiovascular remodeling, and neurodegenerative diseases. Understanding ZAK phosphorylation at Ser165 provides insights into stress-adaptation mechanisms and potential therapeutic targets for diseases involving dysregulated kinase signaling. Proper controls, such as unphosphorylated peptide blocking or phosphatase treatment, are recommended to confirm signal specificity.
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