Rabbit Polyclonal PASK(Phospho-Thr1165) Antibody

The PASK (Phospho-Thr1165) antibody is a specialized tool used to study the phosphorylation status of PAS kinase (PASK), a serine/threonine kinase involved in nutrient sensing and metabolic regulation. PASK contains two N-terminal PAS domains, which sense cellular energy/nutrient status, and a C-terminal kinase domain. Thr1165. located in the kinase activation loop, is a critical phosphorylation site required for PASK activation. Phosphorylation at Thr1165 enhances kinase activity, enabling PASK to regulate processes like glucose homeostasis, lipid metabolism, and protein synthesis by targeting substrates such as AMPK, mTOR, and translation initiation factors. This antibody specifically detects endogenous PASK phosphorylated at Thr1165. allowing researchers to monitor its activation under varying metabolic conditions (e.g., fasting, high glucose) or in disease models like diabetes and obesity. It is commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate PASK’s role in cellular stress responses, mitochondrial function, and insulin signaling pathways. Studies utilizing this antibody have contributed to understanding how nutrient-sensitive kinases adapt cellular metabolism to environmental changes. Proper validation (e.g., knockout controls, peptide competition) is essential to ensure specificity due to potential cross-reactivity with other phospho-epitopes.