| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | STK24, MST-3, MST3, Serine/threonine kinase 24, STE20-like kinase 3, STE20-like kinase MST3, MST3B, STE20, Sterile 20-like kinase 3 |
| Entrez GeneID | 8428; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human MST3 (Phospho-Thr18) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MST3 (Phospho-Thr18)抗体的3篇参考文献示例(注：文献为虚拟示例，实际研究中请查询具体数据库)：
1. **文献名称**：*"MST3 Kinase Activation via Phosphorylation at Thr18 in Response to Oxidative Stress"*
**作者**：Chen et al.
**摘要**：本研究通过Phospho-Thr18抗体检测MST3在氧化应激条件下的激活状态，发现Thr18磷酸化是MST3激酶活性的关键调节机制，并揭示其参与调控细胞凋亡通路。
2. **文献名称**：*"Phosphorylation-Dependent Regulation of MST3 in Cell Cycle Progression"*
**作者**：Rodriguez et al.
**摘要**：利用MST3 (Phospho-Thr18)特异性抗体，作者证实Thr18位点的磷酸化在G1/S期转换中起重要作用，并通过免疫印迹分析验证其与Cyclin D1的相互作用。
3. **文献名称**：*"Role of MST3 Thr18 Phosphorylation in Neuronal Differentiation"*
**作者**：Kimura et al.
**摘要**：通过免疫荧光和Western blot技术，研究发现MST3的Thr18磷酸化水平在神经元分化过程中显著上调，提示其在神经突生长和突触形成中的功能依赖性。
如需具体文献，建议在PubMed、Google Scholar等平台检索关键词“MST3 Phospho-Thr18 antibody”或相关激酶调控研究。
The MST3 (Phospho-Thr18) antibody is a specialized tool used to detect the phosphorylated form of mammalian STE20-like kinase 3 (MST3) at threonine residue 18. MST3. also known as STK24. belongs to the GCK-III subfamily of STE20 kinases and plays critical roles in regulating cell apoptosis, stress response, and polarity. Phosphorylation at Thr18 is a key post-translational modification linked to MST3 activation, which enables its participation in signaling cascades, including the Hippo pathway. This phosphorylation event is thought to modulate MST3’s kinase activity, substrate binding, or subcellular localization, though exact mechanisms remain under investigation.
The antibody is typically generated by immunizing hosts with synthetic peptides corresponding to the phosphorylated Thr18 region, ensuring specificity for the activated form of MST3. It is widely employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study MST3’s activation dynamics in cellular stress, development, or disease contexts. Researchers use it to explore MST3’s role in processes such as neuronal differentiation, tumor suppression, or immune regulation. Validation often includes knockout controls or phosphatase treatment to confirm phosphorylation-dependent signals. Its application aids in understanding how MST3-mediated signaling contributes to physiological and pathological states, offering insights for therapeutic targeting.
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