| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PRKG2, CGK 2, CGMP protein kinase II, CGKII, CGMP-PK type II, PKGII, PRKGR2, CGK2, CGMP kinase II |
| Entrez GeneID | 5593; |
| WB Predicted band size | 87kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PKG2 (Phospho-Ser126) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PKG2 (Phospho-Ser126)抗体的示例参考文献(注：以下内容为虚构示例，实际文献需通过学术数据库查询)：
1. **文献名称**：*Phosphorylation of cGMP-dependent protein kinase II at Ser126 modulates vascular smooth muscle function*
**作者**：Zhang L, et al.
**摘要**：本研究利用PKG2 (Phospho-Ser126)抗体，发现该位点的磷酸化在血管平滑肌细胞舒张中起关键作用，并通过Western blot和免疫荧光验证其在高血压模型中的表达变化。
2. **文献名称**：*Role of PKG2 Ser126 phosphorylation in intestinal mucosal barrier regulation*
**作者**：Kim H, et al.
**摘要**：通过特异性PKG2 (Phospho-Ser126)抗体，揭示了该磷酸化事件通过调控紧密连接蛋白影响肠黏膜通透性，为炎症性肠病治疗提供新靶点。
3. **文献名称**：*Development and validation of a phospho-specific antibody for PKG2 Ser126 in neurodegenerative models*
**作者**：Smith J, et al.
**摘要**：本文报道了一种高特异性PKG2 (Phospho-Ser126)抗体的制备，并证明其在阿尔茨海默病模型中检测PKG2活性变化的可靠性，支持其用于神经退行性病变研究。
4. **文献名称**：*PKG2 phosphorylation at Ser126 mediates cardiac hypertrophy signaling*
**作者**：Wang Y, et al.
**摘要**：利用该抗体发现Ser126磷酸化在心肌细胞肥大信号通路中与cGMP水平动态相关，为心力衰竭的分子机制提供了新见解。
(提示：以上文献为示例，实际引用请通过PubMed、Web of Science等平台检索。)
The PKG2 (Phospho-Ser126) antibody is a specialized tool used to detect the phosphorylated form of cGMP-dependent protein kinase type II (PKG2) at serine residue 126. PKG2. also known as PRKG2. is a key enzyme in cyclic GMP (cGMP) signaling, regulating processes like ion transport, smooth muscle relaxation, and cell proliferation. Its activity is tightly controlled by cGMP binding and post-translational modifications, including phosphorylation. Phosphorylation at Ser126. located in the kinase’s regulatory domain, is associated with conformational changes that modulate PKG2 activation and downstream signaling. This modification may influence enzyme sensitivity to cGMP or interactions with substrates, though its precise functional role remains under investigation.
The Phospho-Ser126 antibody is particularly valuable in studies exploring PKG2 activation dynamics in physiological or pathological contexts, such as cardiovascular function, gastrointestinal motility, or bone remodeling. Researchers employ this antibody in techniques like Western blotting, immunohistochemistry, or immunofluorescence to assess PKG2 phosphorylation status, often correlating it with cGMP pathway activity. Its specificity for the phosphorylated epitope allows differentiation between active and inactive PKG2 forms, aiding mechanistic studies of cGMP-dependent signaling. Validated applications typically include cell or tissue models where PKG2 plays a documented role, with proper controls (e.g., dephosphorylation treatments) recommended to confirm signal specificity. This tool has advanced understanding of PKG2’s role in diseases like hypertension, diabetes, and cancer.
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