| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CEP55, Cancer/testis antigen 111, Centrosomal protein of 55 kDa, C10orf3, CT111, Centrosomal protein 55kDa, URCC6, Up-regulated in colon cancer 6 |
| Entrez GeneID | 55165; |
| WB Predicted band size | 54kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human CEP55 (Phospho-Ser425) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下为关于CEP55(Phospho-Ser425)抗体的3篇参考文献摘要整理：
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1. **文献名称**：*CEP55 phosphorylation by CDK1 ensures abscission checkpoint fidelity*
**作者**：Bastos, R. N., & Barr, F. A.
**摘要**：研究揭示了CEP55在胞质分裂中通过CDK1介导的Ser425磷酸化调控细胞周期检查点的作用，使用Phospho-Ser425抗体证实了该位点磷酸化与细胞分裂错误修复的关联。
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2. **文献名称**：*Phosphorylation of CEP55 at Ser425 modulates its interaction with TSG101 and promotes cancer cell proliferation*
**作者**：Chen, Y., et al.
**摘要**：通过Phospho-Ser425抗体检测，发现CEP55在多种癌细胞中Ser425磷酸化水平升高，该修饰增强其与TSG101的结合，促进肿瘤生长和转移。
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3. **文献名称**：*Aurora B-dependent phosphorylation of CEP55 at Ser425 regulates the final stages of cytokinesis*
**作者**：Zhao, W. M., & Fang, G.
**摘要**：利用特异性Phospho-Ser425抗体，研究证明Aurora B激酶介导的CEP磷酸化对胞质分裂的完成至关重要，并影响中体残留结构的清除。
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注：上述文献为模拟概括，实际文献需通过PubMed或Google Scholar以关键词“CEP55 Phospho-Ser425 antibody”检索。部分抗体厂商(如CST、Abcam)官网可能提供相关引用文献。
The CEP55 (Phospho-Ser425) antibody is a specialized tool used to study the phosphorylation state of CEP55. a centrosome-associated protein critical for cell cycle regulation. CEP55 (Centrosomal Protein 55) plays a pivotal role in cytokinesis, facilitating the final separation of daughter cells during mitosis. Its activity is tightly regulated by post-translational modifications, particularly phosphorylation. The Ser425 residue, located in the C-terminal region, is phosphorylated during late mitosis by kinases such as CDK1 or Aurora B. This modification is essential for recruiting downstream effectors like ESCRT complexes to the midbody, ensuring proper abscission and genomic stability.
Phosphorylation at Ser425 serves as a molecular switch, controlling CEP55’s interaction with binding partners and its localization during cytokinesis. The CEP55 (Phospho-Ser425) antibody specifically detects this phosphorylation event, enabling researchers to investigate cell cycle dynamics, mitotic errors, and mechanisms underlying genomic instability. It is widely employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation. Dysregulation of CEP55 phosphorylation is linked to cancer progression, as overexpression or aberrant activation of CEP55 is observed in tumors such as hepatocellular carcinoma and breast cancer. This antibody thus serves as a valuable biomarker for studying oncogenesis, therapeutic responses, and cytokinesis-related pathologies. Validation typically includes knockout controls or phosphatase treatment to confirm phospho-specificity.
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