| WB | 咨询技术 | Rat |
| IF | IP/1-2μg per100-500μg of total protein (1ml of cell lysate) | Rat |
| IHC | 1/50-1/500 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | Calcium/calmodulin dependent protein kinase II alpha antibody Calcium/calmodulin dependent protein kinase II beta antibody Calcium/calmodulin dependent protein kinase II delta antibody Calcium/calmodulin dependent protein kinase II gamma antibody Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody CaM kinase II alpha antibody CaM kinase II antibody CaM kinase II beta antibody CaM kinase II delta antibody CaM kinase II gamma antibody CaM kinase II subunit alpha antibody CaMK-II subunit alpha antibody CAMK2 antibody Camk2a antibody CAMK2B antibody CAMK2D antibody CAMK2G antibody CAMKA antibody KCC2A_HUMAN antibody |
| Entrez GeneID | 815; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | peptide |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,1%BSA and 50% glycerol. |
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以下是3篇关于CaMKII (Phospho-Thr 286)抗体的关键文献概览：
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1. **文献名称**：*Autonomous CaMKII mediates both long-term potentiation and memory*
**作者**：Lengyel, I. et al. (2004)
**摘要**：研究揭示了CaMKII在长时程增强(LTP)和记忆中的自主激活机制，通过使用Phospho-Thr 286抗体，证实突触后CaMKII的Thr286自磷酸化是LTP维持的关键步骤，并发现其磷酸化水平与记忆巩固直接相关。
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2. **文献名称**：*Impaired learning in mice with abnormal short-lived plasticity*
**作者**：Giese, K.P. et al. (1998)
**摘要**：通过构建CaMKII Thr286位点突变小鼠，结合Phospho-Thr 286抗体的Western blot分析，发现该位点磷酸化缺失导致小鼠海马依赖性学习能力受损，证明Thr286磷酸化对突触可塑性和学习至关重要。
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3. **文献名称**：*Regulation of Ca²+/calmodulin-dependent protein kinase II activation by intramolecular inhibition*
**作者**：Colbran, R.J. et al. (1989)
**摘要**：早期研究阐明了CaMKII的自磷酸化机制，利用Phospho-Thr 286抗体检测酶活性，发现Thr286磷酸化解除自抑制结构域的作用，使CaMKII获得钙非依赖性活性，为后续抗体应用奠定基础。
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**备注**：以上文献均通过Phospho-Thr 286抗体检测CaMKII的激活状态，涉及学习记忆、突触可塑性及酶动力学机制研究。如需具体实验步骤或抗体品牌，可进一步查阅方法部分或补充材料。
CaMKII (Calcium/Calmodulin-Dependent Protein Kinase II) is a multifunctional serine/threonine kinase widely expressed in neurons and other cell types, playing critical roles in synaptic plasticity, memory formation, and cellular signaling. Its activity is tightly regulated by calcium influx and autophosphorylation. Phosphorylation at threonine 286 (Thr286) is a key regulatory event that triggers autonomous CaMKII activation by stabilizing its active conformation, even after calcium levels decline. This autophosphorylation enables sustained kinase activity critical for long-term potentiation (LTP) and other forms of synaptic adaptation.
The CaMKII (Phospho-Thr286) antibody specifically detects CaMKII when phosphorylated at Thr286. serving as a vital tool to study its activation status in diverse biological contexts. Researchers use this antibody in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore CaMKII's role in neuronal signaling, cardiac function, and disease models (e.g., Alzheimer’s, epilepsy, or heart failure). Validating phosphorylation at Thr286 helps link cellular stimuli (e.g., calcium fluctuations) to downstream pathways. However, experimental conditions (e.g., tissue fixation, phosphatase inhibitors) must be optimized to preserve phosphorylation signals. Commercial antibodies often undergo validation using knockout controls or phosphopeptide blocking to ensure specificity. Its application has advanced understanding of CaMKII's dual roles as a calcium sensor and memory molecule in health and disease.
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