| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMR3B |
| Uniprot No | P02814 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-79aa |
| 氨基酸序列 | MKSLTWILGL WALAACFTPG ESQRGPRGPY PPGPLAPPQP FGPGFVPPPP PPPYGPGRIP PPPPAPYGPG IFPPPPPQP |
| 预测分子量 | 7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Expression and Characterization of Recombinant Mouse SMR3B in Escherichia coli"**
- Authors: Zhang Y, Wang L, Chen H
- 摘要：研究利用大肠杆菌系统表达重组小鼠SMR3B蛋白，优化诱导条件并验证其分泌蛋白特性，通过Western blot和质谱确认蛋白正确性。
2. **"Structural Analysis of Recombinant SMR3B and Its Role in Salivary Gland Function"**
- Authors: Tanaka K, Sato R, Yamamoto M
- 摘要：通过哺乳动物细胞表达重组SMR3B，解析其三维结构，发现其与钙离子结合能力，推测其参与唾液润滑及矿物质稳态调控。
3. **"Recombinant SMR3B as a Biomarker for Salivary Gland Disorders in Mouse Models"**
- Authors: Gupta S, Patel T, Lee J
- 摘要：构建重组SMR3B蛋白，应用于唾液腺疾病小鼠模型，证明其在唾液分泌减少症中表达显著下调，提示其作为潜在诊断标记物。
4. **"Comparative Study of SMR3B Recombinant Production in Prokaryotic vs. Eukaryotic Systems"**
- Authors: Müller F, Schmidt D, Weber A
- 摘要：对比大肠杆菌与昆虫细胞表达的重组SMR3B，发现真核系统更利于蛋白糖基化修饰，但原核系统产量更高，适用于功能初步研究。
注：以上文献信息为基于领域知识的模拟示例，实际引用需查询具体数据库(如PubMed、Web of Science)获取真实文献。
**Background of SMR3B Recombinant Protein**
SMR3B (Small Proline-Rich Protein 3B) is a secreted protein encoded by the *SMR3B* gene, predominantly expressed in salivary glands and mucosal tissues. It belongs to the proline-rich protein family, characterized by repetitive proline-rich motifs that contribute to structural stability and functional interactions. SMR3B is synthesized as a precursor protein and undergoes proteolytic processing to release mature peptides, which play roles in mucosal lubrication, innate immunity, and tissue protection.
The recombinant SMR3B protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, enabling controlled production for research. Its structure includes conserved proline-rich domains and potential glycosylation sites, which may influence its binding to microbial surfaces or host receptors. Studies suggest SMR3B contributes to maintaining mucosal barrier integrity by regulating hydration, viscosity, and antimicrobial activity in secretions.
In pathological contexts, altered SMR3B expression has been linked to oral diseases, cystic fibrosis, and cancer. For instance, reduced levels are observed in salivary dysfunction, while overexpression in tumors may correlate with immune evasion. Recombinant SMR3B serves as a tool to study these mechanisms, screen therapeutic agents, or develop biomarkers. Its applications extend to investigating protein-protein interactions, mucosal immunity, and tissue repair pathways.
Overall, SMR3B recombinant protein bridges basic and translational research, offering insights into mucosal biology and potential clinical innovations.
×
