WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 1/100-1/500 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | 28S ribosomal protein S21; mitochondrial; S21mt; MRP-S21; MRPS21 |
Entrez GeneID | 54460; |
WB Predicted band size | 11kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | Synthesized peptide derived from internal of human MRPS21. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MRPS21抗体的示例参考文献(内容为模拟,建议通过学术数据库获取真实文献):
1. **文献名称**: *MRPS21 modulates mitochondrial translation and cell proliferation in colorectal cancer*
**作者**: Zhang L, et al.
**摘要**: 研究通过Western blot和免疫组化分析MRPS21在结直肠癌中的表达,发现其高表达与患者预后不良相关,并证实MRPS21抗体在检测肿瘤中线粒体翻译活性中的作用。
2. **文献名称**: *Loss of MRPS21 impairs mitochondrial ribosome assembly leading to respiratory chain deficiencies*
**作者**: Wang Y, et al.
**摘要**: 利用CRISPR-Cas9敲低MRPS21.结合特异性抗体检测蛋白表达,发现其缺失导致线粒体核糖体组装缺陷,进而影响呼吸链复合物功能。
3. **文献名称**: *MRPS21 mutations cause human mitochondrial disease via disrupted mitochondrial protein synthesis*
**作者**: Metodiev MD, et al.
**摘要**: 通过患者样本和细胞模型,使用MRPS21抗体验证突变导致的蛋白表达异常,揭示其通过破坏线粒体蛋白合成引发多系统线粒体疾病。
4. **文献名称**: *Antibody-based profiling of mitochondrial ribosome proteins in neurodegenerative disorders*
**作者**: Smith J, et al.
**摘要**: 开发针对MRPS21等线粒体核糖体蛋白的抗体库,分析阿尔茨海默病脑组织中该类蛋白的表达变化,提示其与神经元能量代谢障碍相关。
**建议**:通过PubMed或Google Scholar搜索“MRPS21 antibody”、“MRPS21 mitochondrial function”等关键词,筛选近5年文献以获取最新进展。
The mitochondrial ribosomal protein S21 (MRPS21) is a component of the small subunit (28S) of the mitochondrial ribosome, which is essential for translating mitochondrial DNA-encoded mRNAs involved in oxidative phosphorylation (OXPHOS). As a nuclear-encoded protein, MRPS21 is synthesized in the cytoplasm and imported into mitochondria, where it participates in ribosome assembly and protein synthesis. Dysregulation of MRPS21 has been linked to mitochondrial dysfunction, impacting cellular energy production, metabolism, and apoptosis. Studies suggest its potential role in diseases such as cancer, neurodegenerative disorders, and primary mitochondrial diseases, where altered mitochondrial translation machinery contributes to pathology.
MRPS21 antibodies are immunological tools used to detect and quantify MRPS21 expression in research applications like Western blotting, immunofluorescence, and immunohistochemistry. These antibodies aid in investigating mitochondrial ribosome biogenesis, protein synthesis dynamics, and disease mechanisms. For instance, MRPS21 antibodies have been employed to study its downregulation in certain cancers, correlations with poor prognosis, or mutations in mitochondrial disorders. Commercial MRPS21 antibodies are typically raised in rabbits or mice using synthetic peptides or recombinant proteins as immunogens. Validation includes testing specificity via knockout cell lines or siRNA-mediated depletion. Researchers rely on these antibodies to explore mitochondrial translation defects, metabolic adaptations, and therapeutic targets in mitochondrial-associated diseases.
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