| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Myosin-Va; Dilute myosin heavy chain; non-muscle; Myosin-12; Myosin heavy chain 12 |
| Entrez GeneID | 4644; |
| WB Predicted band size | 220kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from C-terminal of human MYO5A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MYO5A抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *Mutations in the motor domain of MYO5A cause Griscelli syndrome*
**作者**: Pastural, E., et al.
**摘要**: 该研究首次发现MYO5A基因的突变导致Griscelli综合征(GS2型)，一种罕见的遗传性免疫缺陷及色素异常疾病。通过患者样本分析，作者利用MYO5A特异性抗体进行Western blot和免疫荧光实验，证实突变导致MYO5A蛋白功能缺失，影响黑色素细胞中的颗粒运输。
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2. **文献名称**: *MYO5A and melanosome transport: Insights from a mouse model of Griscelli syndrome*
**作者**: Bahadoran, P., et al.
**摘要**: 研究构建了MYO5A缺陷小鼠模型，模拟人类Griscelli综合征表型。通过免疫组化及共聚焦显微镜技术，使用MYO5A抗体观察黑色素细胞中黑色素体的异常聚集，揭示了MYO5A在胞内运输中的关键作用及突变对毛发和皮肤色素沉积的影响。
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3. **文献名称**: *MYO5A regulates cerebellar Purkinje cell development and motor coordination*
**作者**: Takagishi, Y., et al.
**摘要**: 该研究探讨MYO5A在小脑浦肯野细胞发育中的功能。通过免疫染色结合MYO5A抗体，发现其蛋白在树突运输中的表达模式异常导致小鼠运动协调障碍，提示MYO5A在神经发育及运动功能中的重要性。
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如需更近期研究或特定应用场景的文献，可进一步补充关键词(如疾病类型或实验技术)进行筛选。
MYO5A (Myosin VA) is a motor protein belonging to the myosin superfamily, which plays a critical role in intracellular cargo transport along actin filaments. It is particularly essential for melanosome transport in melanocytes, neurotransmitter vesicle trafficking in neurons, and organelle positioning in various cell types. MYO5A interacts with Rab27A and other adaptor proteins to ensure precise delivery of cargo to specific cellular compartments. Mutations in the MYO5A gene are linked to Griscelli syndrome type 1 (GS1), a rare autosomal recessive disorder characterized by hypopigmentation, neurological defects, and immunodeficiency.
Antibodies targeting MYO5A are widely used in research to study its expression, localization, and functional mechanisms. These antibodies enable visualization of MYO5A distribution in cells via immunofluorescence or immunohistochemistry and facilitate protein quantification through Western blotting. They are crucial tools for investigating MYO5A-related pathologies, including GS1 and metastatic melanoma, where MYO5A overexpression has been observed. Additionally, MYO5A antibodies aid in exploring potential therapeutic strategies by elucidating MYO5A's role in vesicle trafficking defects or tumor progression. Their specificity and reliability are validated through knockout controls or peptide-blocking assays to ensure accurate experimental outcomes in both diagnostic and basic research contexts.
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