| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NADH dehydrogenase [ubiquinone] flavoprotein 3; mitochondrial; NADH-ubiquinone oxidoreductase 9 kDa subunit; Complex I-9kD; CI-9kD |
| Entrez GeneID | 4731; |
| WB Predicted band size | 120kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human NDUFV3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NDUFV3抗体的模拟参考文献示例(注：以下内容为虚构示例，实际文献需通过学术数据库查询)：
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1. **文献名称**: "Mitochondrial Complex I Dysfunction in Parkinson's Disease: Role of NDUFV3 Subunit"
**作者**: Smith A, et al.
**摘要**: 本研究通过Western blot和免疫组化技术，使用NDUFV3抗体检测帕金森病患者脑组织中复合物I亚基的表达水平，发现NDUFV3显著下调，提示其与线粒体功能障碍相关。
2. **文献名称**: "NDUFV3 Antibody-Based Profiling of Mitochondrial Complex I in Cancer Metabolism"
**作者**: Zhang L, et al.
**摘要**: 利用NDUFV3抗体分析多种癌细胞系的线粒体复合物I组装，发现NDUFV3表达与肿瘤代谢重编程相关，尤其在缺氧条件下其稳定性降低。
3. **文献名称**: "Genetic Mutations in NDUFV3 Disrupt Electron Transport Chain Assembly"
**作者**: Johnson R, et al.
**摘要**: 通过NDUFV3抗体免疫沉淀技术，揭示某些遗传突变导致NDUFV3与其他复合物I亚基结合异常，从而影响线粒体呼吸链功能。
4. **文献名称**: "NDUFV3 Knockdown Exacerbates Cardiac Ischemia-Reperfusion Injury via ROS Overproduction"
**作者**: Lee S, et al.
**摘要**: 使用NDUFV3抗体验证基因敲低模型中线粒体复合物I的缺失，证明NDUFV3缺失会增强氧化应激，导致心肌细胞损伤。
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建议通过PubMed、Google Scholar等平台，以“NDUFV3 antibody”或“NDUFV3 complex I”为关键词检索真实文献。
The NDUFV3 antibody is a crucial tool for studying the NADH:ubiquinone oxidoreductase core subunit V3 (NDUFV3), a component of mitochondrial Complex I in the electron transport chain. Complex I, the largest enzyme in oxidative phosphorylation, facilitates NADH oxidation and ubiquinone reduction, driving ATP synthesis. NDUFV3 is a nuclear-encoded subunit localized to the mitochondrial matrix membrane, contributing to the enzyme’s structural integrity and catalytic activity. Mutations or dysregulation in NDUFV3 have been linked to mitochondrial disorders, such as Leigh syndrome, and are implicated in neurodegenerative diseases, cancer, and metabolic syndromes due to disrupted energy production.
NDUFV3 antibodies enable researchers to investigate protein expression, localization, and post-translational modifications in tissues or cell lines. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess Complex I assembly or dysfunction. These antibodies also aid in diagnosing mitochondrial diseases and exploring therapeutic targets. Commercial NDUFV3 antibodies are typically validated for specificity across species, though variability may exist depending on epitope regions. Their application extends to studying metabolic adaptations in cancer, aging, and genetic models, providing insights into cellular bioenergetics and disease mechanisms.
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