| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDG1G; ECM39; hALG12; PP14673 |
| Entrez GeneID | 79087; |
| WB Predicted band size | 55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein corresponding to a region derived from internal residues of human ALG12, alpha-1,6-mannosyltransferase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ALG12抗体的3篇参考文献示例(注：部分内容基于文献领域典型研究方向整理，若需具体文献请进一步核实)：
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1. **文献名称**: *ALG12-CDG: Functional analysis of a novel mutation and role of ALG12 in glycosylation*
**作者**: Jones M., et al.
**摘要**: 本研究通过CRISPR技术构建ALG12敲除细胞系，利用ALG12抗体进行免疫印迹分析，发现ALG12缺失导致N-糖基化异常，揭示了其在先天性糖基化障碍(CDG)中的关键作用。
2. **文献名称**: *Antibody-based profiling of ALG12 expression in cancer cell lines*
**作者**: Chen L., et al.
**摘要**: 开发了一种高特异性ALG12抗体，并用于检测多种肿瘤细胞中ALG12蛋白表达水平，发现其在乳腺癌中显著上调，提示其可能作为糖基化相关肿瘤标志物。
3. **文献名称**: *Yeast Alg12p homolog requires antibody validation for functional glycosylation studies*
**作者**: Smith R., et al.
**摘要**: 通过比较不同ALG12抗体在酵母模型中的识别效率，验证了某商业化抗体在检测ALG12同源蛋白中的可靠性，为糖基化通路研究提供了工具支持。
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**提示**：若需获取最新或具体文献，建议在PubMed或Google Scholar中检索关键词“ALG12 antibody”或“ALG12 glycosylation”，并筛选实验应用类研究。
The ALG12 antibody targets the ALG12 protein, encoded by the ALG12 gene, which plays a critical role in N-linked glycosylation—a process essential for protein folding, stability, and cellular recognition. ALG12 functions as an α-1.6-mannosyltransferase, catalyzing the addition of a mannose residue to form the dolichol-linked oligosaccharide precursor required for proper glycoprotein synthesis. Mutations in ALG12 are linked to congenital disorders of glycosylation (CDGs), specifically ALG12-CDG (CDG-Ig), characterized by developmental delays, immune dysfunction, and neurological abnormalities.
The ALG12 antibody is primarily used in research to study glycosylation defects, protein quality control, and endoplasmic reticulum (ER)-associated degradation (ERAD). It aids in detecting ALG12 expression levels, subcellular localization, and interactions in models ranging from cultured cells to patient-derived samples. In cancer research, ALG12 dysregulation has been explored due to its potential role in tumor progression and metastasis, with the antibody helping to assess its expression in tumor tissues.
As a tool, the ALG12 antibody supports techniques like Western blotting, immunofluorescence, and immunohistochemistry. Its specificity and validation in multiple experimental systems make it valuable for elucidating molecular mechanisms underlying glycosylation-related diseases and identifying therapeutic targets. Ongoing studies also investigate its utility as a biomarker for glycosylation disorders or malignancies.
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