| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COMP |
| Uniprot No | P49747 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-757aa |
| 氨基酸序列 | QGQSPLGSDL GPQMLRELQE TNAALQDVRE LLRQQVREIT FLKNTVMECD ACGMQQSVRT GLPSVRPLLH CAPGFCFPGV ACIQTESGAR CGPCPAGFTG NGSHCTDVNE CNAHPCFPRV RCINTSPGFR CEACPPGYSG PTHQGVGLAF AKANKQVCTD INECETGQHN CVPNSVCINT RGSFQCGPCQ PGFVGDQASG CQRRAQRFCP DGSPSECHEH ADCVLERDGS RSCVCAVGWA GNGILCGRDT DLDGFPDEKL RCPERQCRKD NCVTVPNSGQ EDVDRDGIGD ACDPDADGDG VPNEKDNCPL VRNPDQRNTD EDKWGDACDN CRSQKNDDQK DTDQDGRGDA CDDDIDGDRI RNQADNCPRV PNSDQKDSDG DGIGDACDNC PQKSNPDQAD VDHDFVGDAC DSDQDQDGDG HQDSRDNCPT VPNSAQEDSD HDGQGDACDD DDDNDGVPDS RDNCRLVPNP GQEDADRDGV GDVCQDDFDA DKVVDKIDVC PENAEVTLTD FRAFQTVVLD PEGDAQIDPN WVVLNQGREI VQTMNSDPGL AVGYTAFNGV DFEGTFHVNT VTDDDYAGFI FGYQDSSSFY VVMWKQMEQT YWQANPFRAV AEPGIQLKAV KSSTGPGEQL RNALWHTGDT ESQVRLLWKD PRNVGWKDKK SYRWFLQHRP QVGYIRVRFY EGPELVADSN VVLDTTMRGG RLGVFCFSQE NIIWANLRYR CNDTIPEDYE THQLRQA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COMP重组蛋白的3篇参考文献示例，涵盖结构、功能及应用研究：
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1. **文献名称**：*"Recombinant Human COMP: Expression, Purification, and Structural Characterization"*
**作者**：Zhang, Y., et al.
**摘要**：本研究在大肠杆菌中成功表达并纯化了重组人源COMP蛋白，通过X射线晶体学解析其三维结构，揭示了其五聚体组装机制及与胶原结合的分子界面，为理解COMP在细胞外基质中的作用提供结构基础。
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2. **文献名称**：*"COMP Enhances Cartilage Regeneration in a Murine Osteoarthritis Model"*
**作者**：Lee, S., & Kim, H.J.
**摘要**：通过体外实验和小鼠模型证明，重组COMP蛋白可显著促进软骨细胞增殖和胶原合成，并抑制炎症因子释放，提示其在骨关节炎治疗中的潜在应用价值。
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3. **文献名称**：*"Mutation-Induced COMP Misfolding Causes Endoplasmic Reticulum Stress in Chondrodysplasias"*
**作者**：Posey, K.L., et al.
**摘要**：研究发现，假性软骨发育不全患者的COMP突变体在重组表达时引发内质网应激，导致软骨细胞凋亡，揭示了COMP蛋白错误折叠与遗传性骨骼疾病的分子关联。
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4. **文献名称**：*"Recombinant COMP as a Biomaterial Scaffold for Tendon Repair"*
**作者**：Müller, S.A., et al.
**摘要**：开发基于重组COMP的生物材料支架，实验显示其能有效支持肌腱细胞黏附和力学强度恢复，为软组织工程提供了新型功能性材料。
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**备注**：以上文献为示例，实际引用时需核实真实文献及作者信息。建议通过PubMed或Web of Science以关键词“recombinant COMP protein”或“cartilage oligomeric matrix protein”检索最新研究。
**Background of COMP Recombinant Protein**
Cartilage Oligomeric Matrix Protein (COMP), a glycoprotein belonging to the thrombospondin family, plays a critical role in extracellular matrix (ECM) organization and cellular interactions. Primarily expressed in cartilage, tendons, and ligaments, COMP facilitates ECM assembly by binding collagen fibrils, fibronectin, and other matrix components, thereby contributing to tissue integrity and mechanical resilience. Its pentameric structure, formed by five identical subunits linked via disulfide bonds, enhances its ability to interact with multiple ECM molecules simultaneously.
Mutations in the *COMP* gene are associated with skeletal disorders like pseudoachondroplasia and multiple epiphyseal dysplasia, characterized by abnormal cartilage development and early-onset osteoarthritis. These pathologies highlight COMP's importance in maintaining cartilage homeostasis.
Recombinant COMP proteins are engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional COMP for research and therapeutic applications. These proteins retain key biological activities, such as binding collagen and modulating chondrocyte behavior, making them valuable tools for studying ECM dynamics, disease mechanisms, and tissue engineering.
In regenerative medicine, COMP recombinant proteins are explored for enhancing scaffold-based cartilage repair or promoting stem cell differentiation. Additionally, they serve as biomarkers in osteoarthritis research, as elevated COMP levels in synovial fluid correlate with cartilage degradation.
Overall, COMP recombinant proteins bridge basic research and clinical innovation, offering insights into ECM biology and potential therapies for connective tissue disorders.
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