| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALOX5 |
| Uniprot No | P09917 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-674aa |
| 氨基酸序列 | MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPYYYLSPDRIPNSVAI |
| 分子量 | 104.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人多不饱和脂肪酸5-脂氧合酶(ALOX5)的3-4篇参考文献及其摘要内容的简要整理：
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1. **文献名称**：*ALOX5 is required for the survival of leukemia stem cells in acute myeloid leukemia*
**作者**：Chen Y, Hu Y, Zhang H, et al.
**摘要**：该研究发现ALOX5在急性髓系白血病(AML)的干细胞中高表达，并通过调控脂质过氧化反应维持白血病干细胞的自我更新和耐药性。抑制ALOX5可显著减少白血病干细胞的存活，提示其作为AML治疗靶点的潜力。
2. **文献名称**：*5-Lipoxygenase contributes to the pathogenesis of atherosclerosis by regulating arterial monocyte and macrophage dynamics*
**作者**：Spanbroek R, Grabner R, Lotzer K, et al.
**摘要**：研究通过载脂蛋白E(ApoE)敲除小鼠模型，发现ALOX5通过促进单核细胞趋化与巨噬细胞泡沫化加速动脉粥样硬化斑块形成，ALOX5缺失可降低斑块炎症和脂质沉积，提示其在动脉粥样硬化中的关键作用。
3. **文献名称**：*5-Lipoxygenase regulates tau phosphorylation and synaptic dysfunction in Alzheimer's disease models*
**作者**：Chu J, Li JG, Praticò D.
**摘要**：该研究揭示了ALOX5在阿尔茨海默病(AD)模型中通过激活p38 MAPK/cdk5通路促进tau蛋白过度磷酸化及神经元损伤，抑制ALOX5可改善突触功能障碍和认知衰退，为AD治疗提供了新方向。
4. **文献名称**：*Structural basis of 5-lipoxygenase inhibition by natural products and synthetic compounds*
**作者**：Rådmark O, Werz O, Steinhilber D, et al.
**摘要**：本文系统综述了ALOX5的三维结构、催化机制及其抑制剂(如齐留通和多酚类化合物)的作用方式，强调其在抗炎(如哮喘)和抗癌药物开发中的应用前景。
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以上文献分别从白血病干细胞、动脉粥样硬化、神经退行性疾病及药物开发角度探讨ALOX5的生理病理功能，覆盖其基础研究与临床转化意义。如需具体文献来源，可进一步提供期刊卷号或DOI信息。
**Background of Recombinant Human Polyunsaturated Fatty Acid 5-Lipoxygenase (ALOX5)**
5-Lipoxygenase (ALOX5) is a pivotal enzyme in the metabolism of polyunsaturated fatty acids, particularly arachidonic acid, catalyzing the initial steps in the biosynthesis of pro-inflammatory leukotrienes (LTs). It oxygenates arachidonic acid at the C5 position to form 5-hydroperoxyeicosatetraenoic acid (5-HPETE), which is further converted to leukotriene A4 (LTA4). LTA4 serves as a precursor for other inflammatory mediators, such as LTB4 and cysteinyl LTs (LTC4. LTD4. LTE4), which play critical roles in immune responses, inflammation, and allergic disorders.
ALOX5 is a 78-kDa protein containing two structural domains: an N-terminal β-barrel for membrane interaction and a C-terminal catalytic domain with non-heme iron. Its activity is calcium-dependent and regulated by cellular localization and interactions with co-factors like 5-lipoxygenase-activating protein (FLAP). Dysregulation of ALOX5 is implicated in asthma, cardiovascular diseases, cancer, and neurodegenerative conditions, making it a therapeutic target.
Recombinant human ALOX5. produced via heterologous expression systems (e.g., *E. coli* or insect cells), enables structural and functional studies, inhibitor screening, and mechanistic research. Challenges in producing active recombinant ALOX5 include maintaining solubility and proper folding. Despite this, recombinant ALOX5 remains crucial for developing inhibitors (e.g., zileuton) and understanding its role in disease pathways.
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