| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALS2CR11 |
| Uniprot No | Q53TS8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-623aa |
| 氨基酸序列 | MEPPQETNRPFSTLDNRSGQVQVLSATPLLQRNPYSSPDIMHIKGSEASSVPYALNQGTTALPKNKNQEGTGHRLLNMLRKTLKESDSEELEITQETPNLVPFGDVVGCLGIHIKNCRHFMPKISLQHYANLFIRISINKAVKCTKMCSLLSKNDEKNTVIKFDEVKYFSVQVPRRYDDKRNNILLELIQYDNREKRAFLLGSVQIHLYEVIQKGCFIEEVQVLHGNIFVCRLEVEFMFSYGNFGYGFSHQLKPLQKITEPSMFMNLAPPPERTDPVTKVITPQTVEYPAFLSPDLNVTVGTPAVQSSNQPSVVRLEKLQQQPRERLEKMKKEYRNLNTWIDKANYLESILMPKLEHKDSEETNIDEASENTKSNQPEEELENIVGVDIPLVNEEAETTANELLDNDSEKGLTIPTLNQSDQDNSTADASKNDESTPSPTEVHSLCTISNQETIKAGRIPPLGERQSESMPDRKMKNVFFPLEVKLKDNYPSILKADSSLSEVAFSPKEYNSPSFRPEYIEFKPKFQDCSDKFEDLHDMTSFTHLKKVKSRSRLLGKSSDDIHNHARHSARPYTAPEVNKQRESYSGKFTSRRMVSSGLVHINDKTSDYEMHKMRPKKIKRGY |
| 分子量 | 97.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ALS2CR11蛋白的模拟参考文献示例(注：因该蛋白研究较少，以下内容为假设性示例，建议通过数据库如PubMed核实真实文献)：
1. **《ALS2CR11在神经退行性疾病中的功能研究》**
- 作者：Li X, Zhang Y, et al.
- 摘要：研究报道了ALS2CR11蛋白在神经细胞中的表达及其与ALS2蛋白家族的相互作用，提出其可能通过调控囊泡运输参与肌萎缩侧索硬化症(ALS)相关通路。
2. **《重组ALS2CR11蛋白的异源表达及结构分析》**
- 作者：Wang H, Chen L.
- 摘要：利用大肠杆菌系统成功表达并纯化重组人ALS2CR11蛋白，通过X射线晶体学解析其三维结构，发现其具有独特的GTPase结合结构域。
3. **《ALS2CR11在细胞自噬中的调控作用》**
- 作者：Kim S, Park JH.
- 摘要：通过RNA干扰实验证实，ALS2CR11蛋白的缺失会抑制自噬小体形成，提示其在细胞自噬和线粒体稳态中的关键作用。
4. **《ALS2CR11基因突变与遗传性痉挛性截瘫的关联》**
- 作者：García-Santiago R, et al.
- 摘要：在家族性痉挛性截瘫患者中鉴定出ALS2CR11的罕见错义突变，功能实验表明突变体蛋白导致轴突运输功能障碍。
建议通过PubMed或Google Scholar检索关键词“ALS2CR11 protein”或“ALS2CR11 recombinant”获取最新研究进展。
**Background of Recombinant Human ALS2CR11 Protein**
The recombinant human ALS2CR11 (Amyotrophic Lateral Sclerosis 2 Chromosomal Region Candidate 11) protein is encoded by the *ALS2CR11* gene, located on chromosome 2q33.1. Although its precise biological function remains under investigation, ALS2CR11 is speculated to play roles in cellular processes such as signal transduction, protein-protein interactions, or intracellular trafficking, potentially linked to its structural domains. The protein contains WD40 repeats, motifs commonly involved in scaffold formation and molecular recognition.
Interest in ALS2CR11 stems partly from its genomic proximity to *ALS2*, a gene associated with juvenile amyotrophic lateral sclerosis (ALS), though direct pathological involvement remains unconfirmed. Recombinant production of ALS2CR11 enables functional studies, aiding in elucidating its physiological and pathological relevance. Expressed in systems like *E. coli* or mammalian cells, the purified protein is utilized for biochemical assays, antibody development, and interactome mapping.
Current research focuses on characterizing its interactions with signaling pathways, particularly those implicated in neurodegeneration or cellular stress responses. Despite limited clinical data, ALS2CR11's conserved sequence across species suggests evolutionary importance. Its recombinant form serves as a critical tool for unraveling molecular mechanisms and potential therapeutic targets, though further studies are needed to define its role in health and disease.
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