| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALS2CR8 |
| Uniprot No | Q8N187 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-718aa |
| 氨基酸序列 | MEQSNDSLRVNHNDGEESKTSAQVFEHLICMDSRDSSFGQNDSPTVLPITTREANNSLISQNIPGPLTQTQTLSAEQFHLVDQNGQAIQYELQSLGESNAQMMIVASPTENGQVLRVIPPTQTGMAQVIIPQGQLVDVNSPRDVPEEKPSNRNLPTVRVDTLADNTSNYILHPQTSFPLPKKSVTGMLEEPLLGPLQPLSSNTPIWACRLRSCEKIGDSYRGYCVSETELESVLTFHKQQTQSVWGTRQSPSPAKPATRLMWKSQYVPYDGIPFVNAGSRAVVMECQYGPRRKGFQLKKVSEQESRSCQLYKATCPARIYIKKVQKFPEYRVPTDPKIDKKIIRMEQEKAFNMLKKNLVDAGGVLRWYVQLPTQQAHQYHELETPCLTLSPSPFPVSSLEEEETAVRDENCALPSRLHPQVAHKIQELVSQGIEQVYAVRKQLRKFVERELFKPDEVPERHNLSFFPTVNDIKNHIHEVQKSLRNGDTVYNSEIIPATLQWTTDSGNILKETMTVTFAEGNSPGESITTKVETNQTRGSLSPEPTHLLSSLSSFQPKIFTQLQGLQLQPRYTSPDESPAVVSVNNQPSSSPSGLLDTIGSAVMNNNSLLLGQSHSLQRDTCLTQNNSTASTMGNLPEPDQNLVAMDELVEVGDVEDTGNLEGTVHRILLGDVQTIPIQIIDNHSALIEENPESTISVSQVKQEPKEPALSMEAKNCGL |
| 分子量 | 106.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ALS2CR8蛋白的三条模拟参考文献示例，内容基于学术研究的常见方向虚构而成，供参考格式：
1. **"Cloning and Functional Characterization of Recombinant Human ALS2CR8 in Neuronal Cell Survival"**
*Authors: Smith, J.R., et al.*
**摘要**：本研究成功克隆并表达重组人ALS2CR8蛋白，发现其通过调控RhoGTP酶活性促进神经元存活，可能在与ALS相关的信号通路中发挥关键作用。
2. **"Structural Insights into ALS2CR8’s Role in Vesicle Trafficking via Cryo-EM Analysis"**
*Authors: Li, X., & Wang, H.*
**摘要**：利用冷冻电镜解析ALS2CR8的三维结构，揭示其与Rab5效应蛋白的互作机制，表明该蛋白参与内体运输过程，为神经退行性疾病机制提供新视角。
3. **"ALS2CR8 Knockdown Exacerbates Oxidative Stress-Induced Apoptosis in Motor Neurons"**
*Authors: García, M., et al.*
**摘要**：通过基因沉默实验证明，ALS2CR8缺失加剧运动神经元氧化应激损伤，提示其作为潜在神经保护靶点在ALS治疗中的应用价值。
注：以上文献及作者为虚构示例，实际研究中请以真实数据库(如PubMed)检索为准。
**Background of Recombinant Human ALS2CR8 Protein**
The ALS2CR8 protein, encoded by the *ALS2CR8* (ALS2 C-terminal like receptor 8) gene, is a less characterized member of the RhoGAP-like protein family. It shares homology with the C-terminal region of alsin, a protein linked to amyotrophic lateral sclerosis (ALS), suggesting potential roles in intracellular signaling or vesicle trafficking. Structurally, ALS2CR8 contains conserved domains implicated in GTPase regulation and protein-protein interactions.
Recombinant human ALS2CR8 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Though its precise biological functions remain unclear, ALS2CR8 is hypothesized to modulate small GTPases like Rab5 or Rac1. potentially influencing cytoskeletal dynamics, membrane transport, or neuronal maintenance. Dysregulation of such pathways is associated with neurodegenerative diseases, including ALS, making ALS2CR8 a subject of interest in neurodegeneration research.
Current studies focus on elucidating its interactome, subcellular localization, and role in cellular homeostasis. Further insights into ALS2CR8 could enhance understanding of ALS pathogenesis and broader GTPase-mediated regulatory mechanisms.
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