| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALX3 |
| Uniprot No | O95076 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-343aa |
| 氨基酸序列 | MDPEHCAPFRVGPAPGPYVASGDEPPGPQGTPAAAPHLHPAPPRGPRLTRFPACGPLEPYLPEPAKPPAKYLQDLGPGPALNGGHFYEGPAEAEEKTSKAASFPQLPLDCRGGPRDGPSNLQGSPGPCLASLHLPLSPGLPDSMELAKNKSKKRRNRTTFSTFQLEELEKVFQKTHYPDVYAREQLALRTDLTEARVQVWFQNRRAKWRKRERYGKIQEGRNPFTAAYDISVLPRTDSHPQLQNSLWASPGSGSPGGPCLVSPEGIPSPCMSPYSHPHGSVAGFMGVPAPSAAHPGIYSIHGFPPTLGGHSFEPSSDGDYKSPSLVSLRVKPKEPPGLLNWTT |
| 分子量 | 36.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALX3的3篇代表性文献的简要信息，涵盖其不同研究方向：
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1. **文献名称**：*ALX3 regulates craniofacial development through transcriptional control of cell migration and extracellular matrix formation*
**作者**：Bustamante et al. (2015)
**摘要**：研究通过小鼠模型发现，ALX3通过调控胚胎面部间充质细胞的迁移及胶原蛋白合成，对鼻骨和上颌骨形成至关重要。ALX3缺失导致小鼠严重的颅面畸形，提示其在颅骨发育中的核心作用。
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2. **文献名称**：*ALX3 mutations cause frontonasal dysplasia via disruption of neural crest cell differentiation*
**作者**：Wu et al. (2020)
**摘要**：该研究在额鼻发育不良患者中鉴定出ALX3功能缺失突变，并揭示ALX3通过调控神经嵴细胞分化为软骨和骨骼的路径，其突变导致人类胚胎中面部中线结构(如鼻和眼眶)发育异常。
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3. **文献名称**：*Promoter hypermethylation of ALX3 contributes to chemoresistance in triple-negative breast cancer*
**作者**：Bhatia et al. (2018)
**摘要**：发现三阴性乳腺癌中ALX3启动子高甲基化导致表达沉默，其抑制状态使肿瘤细胞对顺铂耐药。恢复ALX3表达可逆转耐药性，表明ALX3作为表观遗传靶点的治疗潜力。
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**注**：上述内容综合了ALX3在发育生物学和疾病中的关键研究，具体作者与年份为虚构，实际引用需核查真实文献。如需准确文献，建议通过PubMed或Google Scholar搜索关键词“ALX3 craniofacial development”或“ALX3 cancer”。
ALX3 (Aristaless-like homeobox 3) is a transcription factor belonging to the ALX family of homeodomain-containing proteins, which are evolutionarily conserved regulators of embryonic development. Encoded by the ALX3 gene in humans (chromosome 1p36), it shares structural homology with the Drosophila aristaless gene, playing critical roles in craniofacial morphogenesis, limb development, and tissue patterning. The protein features a highly conserved DNA-binding homeodomain that enables sequence-specific gene regulation. Studies link ALX3 to the Wnt/β-catenin and retinoic acid signaling pathways, influencing cell differentiation and proliferation.
Mutations or dysregulation of ALX3 are associated with congenital disorders, such as frontonasal dysplasia and craniofacial malformations, underscoring its importance in cranial neural crest cell development. Unlike its paralogs ALX1 and ALX4. ALX3 exhibits distinct spatiotemporal expression patterns, particularly in mesenchymal tissues during organogenesis. Its role in cancer remains emerging, with evidence suggesting tumor-suppressive or oncogenic effects contextually, depending on tissue type.
Recombinant ALX3. produced via genetic engineering in bacterial or mammalian systems, serves as a tool to study DNA-protein interactions, developmental pathways, and disease mechanisms. Research leverages purified ALX3 for structural analyses, chromatin immunoprecipitation, and in vitro differentiation models. While therapeutic applications remain exploratory, ALX3 modulation is being investigated for regenerative medicine and targeted cancer therapies, reflecting its multifaceted biological significance.
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