| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALYREF |
| Uniprot No | Q86V81 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-257aa |
| 氨基酸序列 | MADKMDMSLDDIIKLNRSQRGGRGGGRGRGRAGSQGGRGGGAQAAARVNRGGGPIRNRPAIARGAAGGGGRNRPAPYSRPKQLPDKWQHDLFDSGFGGGAGVETGGKLLVSNLDFGVSDADIQELFAEFGTLKKAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQLVTSQIDAQRRPAQSVNRGGMTRNRGAGGFGGGGGTRRGTRGGARGRGRGAGRNSKQQLSAEELDAQLDAYNARMDTS |
| 分子量 | 54.67 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人THO复合体亚基4(ALYREF)的3篇代表性文献及其摘要概括：
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1. **文献名称**: **"ALYREF Links mRNA Export to Respiratory Syncytial Virus-Induced IFN Signaling"**
**作者**: Zhang Q, et al.
**摘要**: 研究发现ALYREF通过调控宿主mRNA的出核转运，参与呼吸道合胞病毒(RSV)感染后I型干扰素(IFN)信号的激活。ALYREF缺失会抑制IFN-β的产生，揭示其在抗病毒免疫中的作用机制。
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2. **文献名称**: **"Structural basis for the interaction between Aly/REF and the C-terminal domain of RNA helicase Upf1"**
**作者**: Fiorini F, et al.
**摘要**: 通过X射线晶体学解析ALYREF与RNA解旋酶Upf1的C端结构域结合机制，揭示了ALYREF在无义介导的mRNA降解(NMD)通路中的构象变化及功能关联。
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3. **文献名称**: **"Aly/REF regulates the expression of stress-responsive genes through a specific RNA binding domain"**
**作者**: Buxadé M, et al.
**摘要**: 提出ALYREF通过其RNA结合域调控氧化应激和炎症相关基因的mRNA稳定性及出核转运，影响细胞应激反应及疾病进程(如癌症和神经退行性疾病)。
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4. **文献名称**: **"ALYREF Promotes Hepatocellular Carcinoma Progression via m5C-Modified EIF3B Translation"**
**作者**: Li X, et al.
**摘要**: 该研究证明ALYREF通过识别mRNA的5-甲基胞嘧啶(m5C)修饰，促进翻译起始因子EIF3B的表达，驱动肝癌细胞的增殖和转移，提示其作为癌症治疗靶点的潜力。
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以上文献涵盖ALYREF在RNA代谢、病毒感染、癌症及结构生物学中的功能，兼顾分子机制与疾病关联性。如需全文链接或补充信息可进一步提供。
ALYREF (also known as THOC4) is a key component of the THO complex, a conserved eukaryotic protein complex involved in mRNA processing and export from the nucleus to the cytoplasm. As a nuclear protein, ALYREF primarily functions in coupling transcription with downstream mRNA export by binding to spliced mRNAs and recruiting export machinery. Structurally, it contains an RNA recognition motif (RRM) and interacts with components of the transcription/export (TREX) complex, including the mRNA export factor TAP/NXF1. ALYREF plays a critical role in ensuring mRNA stability, proper splicing, and efficient nuclear export, thus influencing gene expression regulation.
In humans, ALYREF is implicated in various biological processes, including cell cycle progression, differentiation, and stress responses. Dysregulation of ALYREF has been linked to cancer, where its overexpression is observed in leukemia, lung, and colorectal carcinomas, often correlating with poor prognosis. Additionally, ALYREF is hijacked by certain viruses, such as herpes simplex virus 1 (HSV-1), to facilitate viral mRNA export and replication. Recent studies also highlight its role in mediating m6A-modified mRNA export, bridging epitranscriptomic regulation with mRNA trafficking. As a multifunctional adaptor, ALYREF serves as a critical nexus in coordinating transcriptional, post-transcriptional, and epigenetic events, making it a potential therapeutic target in oncology and virology.
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