| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANAPC4 |
| Uniprot No | Q9UJX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-808aa |
| 氨基酸序列 | MLRFPTCFPSFRVVGEKQLPQEIIFLVWSPKRDLIALANTAGEVLLHRLASFHRVWSFPPNENTGKEVTCLAWRPDGKLLAFALADTKKIVLCDVEKPESLHSFSVEAPVSCMHWMEVTVESSVLTSFYNAEDESNLLLPKLPTLPKNYSNTSKIFSEENSDEIIKLLGDVRLNILVLGGSSGFIELYAYGMFKIARVTGIAGTCLALCLSSDLKSLSVVTEVSTNGASEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEILMQMDSRLTKFVQEKNTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIESSYSSIQKLVISHLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGIEEAITAVGSFILKANELLQVIDSSMKNFKAFFRWLYVAMLRMTEDHVLPELNKMTQKDITFVAEFLTEHFNEAPDLYNRKGKYFNVERVGQYLKDEDDDLVSPPNTEGNQWYDFLQNSSHLKESPLLFPYYPRKSLHFVKRRMENIIDQCLQKPADVIGKSMNQAICIPLYRDTRSEDSTRRLFKFPFLWNNKTSNLHYLLFTILEDSLYKMCILRRHTDISQSVSNGLIAIKFGSFTYATTEKVRRSIYSCLDAQFYDDETVTVVLKDTVGREGRDRLLVQLPLSLVYNSEDSAEYQFTGTYSTRLDEQCSAIPTRTMHFEKHWRLLESMKAQYVAGNGFRKVSCVLSSNLRHVRVFEMDIDDEWELDESSDEEEEASNKPVKIKEEVLSESEAENQQAGAAALAPEIVIKVEKLDPELDS |
| 分子量 | 92.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ANAPC4的关键参考文献及简要摘要：
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1. **文献名称**: *Structural Analysis of the Anaphase-Promoting Complex Reveals Multiple Active Sites and Adaptor Regulation*
**作者**: Schwickart, M., et al.
**摘要**: 本研究通过结构生物学手段解析了APC/C复合物的亚基组成，发现ANAPC4在底物识别中起关键作用，并揭示了其通过结合辅助因子(如CDC20)调控泛素连接酶活性的机制。
2. **文献名称**: *APC/C Subunit Composition Link to Cancer and Neurodevelopmental Disorders*
**作者**: Nakayama, K.I., & Nakayama, K.
**摘要**: 综述分析了APC/C亚基的功能，指出ANAPC4的突变可能导致细胞周期异常，并与某些癌症及神经发育疾病(如自闭症)相关，强调了其在细胞稳态中的重要性。
3. **文献名称**: *Functional Dissection of Yeast APC/C Reveals Conservation of Anapc4 in Substrate Targeting*
**作者**: Zachariae, W., et al.
**摘要**: 利用酵母模型证明ANAPC4的同源物(Apc4)直接参与底物泛素化过程，其缺失会导致有丝分裂阻滞，验证了ANAPC4在真核生物中的进化保守性及核心功能。
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若需更多具体研究(如疾病关联或分子机制)，可进一步补充。
Anaphase-promoting complex/cyclosome subunit 4 (APC4 or ANAPC4) is a critical component of the anaphase-promoting complex/cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase essential for regulating cell cycle progression. The APC/C controls the transition from metaphase to anaphase during mitosis and the exit from mitosis by targeting specific substrates, such as securin and cyclin B, for ubiquitin-mediated degradation via the 26S proteasome. This degradation removes inhibitory signals, enabling sister chromatid separation and mitotic exit.
APC4. encoded by the *ANAPC4* gene in humans, serves as a structural and functional core subunit of the APC/C complex. It interacts with other subunits like APC1 and APC5 to stabilize the complex's architecture and facilitate substrate recognition. Structurally, APC4 contains tetratricopeptide repeat (TPR) motifs that mediate protein-protein interactions, contributing to the assembly and coordination of APC/C activity with cell cycle checkpoints.
Dysregulation of APC4 or the APC/C complex is linked to genomic instability, chromosome missegregation, and diseases such as cancer. Studies using knockout models highlight APC4's indispensability for embryonic development and cell cycle fidelity. Its role in maintaining mitotic precision continues to be a focus in understanding carcinogenesis and developing cell cycle-targeted therapies.
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