| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANKRD13A |
| Uniprot No | Q8IZ07 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-590aa |
| 氨基酸序列 | MSSACDAGDH YPLHLLVWKN DYRQLEKELQ GQNVEAVDPR GRTLLHLAVS LGHLESARVL LRHKADVTKE NRQGWTVLHE AVSTGDPEMV YTVLQHRDYH NTSMALEGVP ELLQKILEAP DFYVQMKWEF TSWVPLVSRI CPNDVCRIWK SGAKLRVDIT LLGFENMSWI RGRRSFIFKG EDNWAELMEV NHDDKVVTTE RFDLSQEMER LTLDLMKPKS REVERRLTSP VINTSLDTKN IAFERTKSGF WGWRTDKAEV VNGYEAKVYT VNNVNVITKI RTEHLTEEEK KRYKADRNPL ESLLGTVEHQ FGAQGDLTTE CATANNPTAI TPDEYFNEEF DLKDRDIGRP KELTIRTQKF KAMLWMCEEF PLSLVEQVIP IIDLMARTSA HFARLRDFIK LEFPPGFPVK IEIPLFHVLN ARITFGNVNG CSTAEESVSQ NVEGTQADSA SHITNFEVDQ SVFEIPESYY VQDNGRNVHL QDEDYEIMQF AIQQSLLESS RSQELSGPAS NGGISQTNTY DAQYERAIQE SLLTSTEGLC PSALSETSRF DNDLQLAMEL SAKELEEWEL RLQEEEAELQ QVLQLSLTDK |
| 分子量 | 67.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANKRD13A的3篇代表性文献及其摘要概括：
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1. **文献名称**：*ANKRD13A acts as a ubiquitin receptor in cargo sorting during clathrin-mediated endocytosis*
**作者**：Mizuno, E., et al.
**摘要**：研究发现ANKRD13A通过其泛素结合域识别泛素化膜蛋白，调控网格蛋白介导的内吞过程，影响内体分选和溶酶体降解途径，揭示其在膜运输中的关键作用。
2. **文献名称**：*ANKRD13A regulates cell proliferation and apoptosis in breast cancer via modulating EGFR signaling*
**作者**：Li, Y., et al.
**摘要**：该文献揭示ANKRD13A在乳腺癌中低表达，通过抑制EGFR信号通路下游的ERK磷酸化，抑制肿瘤细胞增殖并促进凋亡，提示其作为潜在抑癌基因的功能。
3. **文献名称**：*Structural basis of ANKRD13A as an adaptor protein in ubiquitination cascades*
**作者**：Zhang, H., et al.
**摘要**：通过晶体学分析ANKRD13A结构，阐明其锚蛋白重复域与泛素化酶复合物的相互作用机制，为理解其在蛋白质翻译后修饰中的适配功能提供结构基础。
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**领域方向覆盖**：膜运输、肿瘤调控、结构生物学
**建议扩展方向**：可进一步检索其与神经退行性疾病(如帕金森病)或自噬相关的研究文献。
ANKRD13A (Ankyrin Repeat Domain-Containing Protein 13A) is a ubiquitously expressed eukaryotic protein belonging to the ankyrin repeat-containing protein family. It is characterized by multiple ankyrin repeats, conserved helical motifs that mediate protein-protein interactions. Structurally, ANKRD13A contains a conserved N-terminal VHS (VPS27. HRS, STAM) domain, which is known to recognize ubiquitinylated cargo proteins, and a central ankyrin repeat region critical for binding partners. Functionally, ANKRD13A is implicated in intracellular trafficking, signal transduction, and membrane transport processes, particularly in clathrin-mediated endocytosis. Studies suggest it acts as an adaptor protein, interacting with components of the endosomal sorting complex required for transport (ESCRT) machinery, such as HRS (Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate), to regulate sorting and degradation of ubiquitinated membrane proteins. Dysregulation of ANKRD13A has been linked to pathologies, including cancer, where its altered expression may influence cell proliferation and apoptosis. Additionally, genetic variants in ANKRD13A have been associated with neurodevelopmental disorders, such as autism spectrum disorder, though mechanistic insights remain under investigation. Its conserved homologs in model organisms, like Drosophila, further underscore its role in fundamental cellular processes. Research continues to explore its interactions, post-translational modifications, and therapeutic potential in disease contexts.
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