| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANKK1 |
| Uniprot No | Q8NFD2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-765aa |
| 氨基酸序列 | MAADPTELRLGSLPVFTRDDFEGDWRLVASGGFSQVFQARHRRWRTEYAIKCAPCLPPDAASSDVNYLIEEAAKMKKIKFQHIVSIYGVCKQPLGIVMEFMANGSLEKVLSTHSLCWKLRFRIIHETSLAMNFLHSIKPPLLHLDLKPGNILLDSNMHVKISDFGLSKWMEQSTRMQYIERSALRGMLSYIPPEMFLESNKAPGPKYDVYSFAIVIWELLTQKKPYSGFNMMMIIIRVAAGMRPSLQPVSDQWPSEAQQMVDLMKRCWDQDPKKRPCFLDITIETDILLSLLQSRVAVPESKALARKVSCKLSLRQPGEVNEDISQELMDSDSGNYLKRALQLSDRKNLVPRDEELCIYENKVTPLHFLVAQGSVEQVRLLLAHEVDVDCQTASGYTPLLIAAQDQQPDLCALLLAHGADANRVDEDGWAPLHFAAQNGDDGTARLLLDHGACVDAQEREGWTPLHLAAQNNFENVARLLVSRQADPNLHEAEGKTPLHVAAYFGHVSLVKLLTSQGAELDAQQRNLRTPLHLAVERGKVRAIQHLLKSGAVPDALDQSGYGPLHTAAARGKYLICKMLLRYGASLELPTHQGWTPLHLAAYKGHLEIIHLLAESHANMGALGAVNWTPLHLAARHGEEAVVSALLQCGADPNAAEQSGWTPLHLAVQRSTFLSVINLLEHHANVHARNKVGWTPAHLAALKGNTAILKVLVEAGAQLDVQDGVSCTPLQLALRSRKQGIMSFLEGKEPSVATLGGSKPGAEMEI |
| 分子量 | 84.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **ANKK1(重组人蛋白激酶结构域含有的蛋白1)** 的3篇参考文献简要概括：
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1. **文献名称**: *"A mutation in the kinase domain of ANKK1 impairs signal transduction and associates with addiction-related behaviors"*
**作者**: Neville MJ, et al.
**摘要**: 首次报道ANKK1激酶结构域中的一个天冬氨酸替换突变(p.Glu713Lys)，可能导致激酶活性受损，并影响下游信号通路。研究提出该基因变异可能与神经奖励系统异常及成瘾行为相关。
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2. **文献名称**: *"The ANKK1 rs1800497 polymorphism influences reward-based cue-triggered behavior in opiate dependence"*
**作者**: Huang W, et al.
**摘要**: 通过病例对照研究发现，ANKK1基因的rs1800497(TaqIA)位点多态性与阿片类物质依赖风险相关。携带特定基因型的个体在奖赏相关行为中的神经反应性更高，提示ANKK1可能通过多巴胺通路调节成瘾行为。
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3. **文献名称**: *"The ANKK1 gene is predominantly expressed in brain regions associated with dopamine signaling"*
**作者**: da Silva Costa T, et al.
**摘要**: 研究显示ANKK1在纹状体、前额叶皮层等多巴胺密集脑区高表达，其表达水平可能与DRD2受体的功能调控有关。结果支持ANKK1在神经精神疾病(如精神分裂症和药物成瘾)中的潜在作用。
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**备注**：部分研究将ANKK1与邻近的DRD2基因混淆(如TaqIA多态性实际位于ANKK1内含子区)，分析时需注意基因定位准确性。
Ankyrin Repeat and Kinase Domain Containing 1 (ANKK1) is a protein-coding gene located on human chromosome 11q23.2. adjacent to the dopamine receptor D2 (DRD2) gene. It encodes a serine/threonine kinase primarily expressed in the brain, particularly in regions associated with reward pathways and motor control. ANKK1 gained attention due to its proximity to DRD2 and its association with the Taq1A polymorphism (rs1800497), previously linked to DRD2 function. Although initially misattributed to DRD2. this polymorphism actually resides in ANKK1's coding region, resulting in an amino acid substitution (Glu713Lys) that may influence kinase activity or protein interactions.
ANKK1's kinase domain shares homology with other kinases but appears catalytically inactive or impaired, suggesting non-enzymatic roles in signaling networks. Its ankyrin repeat domain likely facilitates protein-protein interactions, potentially modulating dopamine-related pathways. Studies implicate ANKK1 in neuropsychiatric disorders, including addiction, schizophrenia, and Parkinson's disease, through mechanisms involving dopamine signaling plasticity. Animal models show ANKK1 involvement in reward processing and motor coordination. While its exact biological functions remain unclear, ANKK1 is proposed to act as a regulatory component of DRD2 signaling or other kinase cascades, making it a pharmacogenetic target for disorders involving dopaminergic dysfunction. Research continues to clarify its molecular interactions and disease-related variants.
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