| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANKMY1 |
| Uniprot No | Q9P2S6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-720aa |
| 氨基酸序列 | MEGAHASLSLEDEVSGAGSRQRPLEGKGGETPAAEEPGSLKNYAVFATRDVSAAPEKEEEEAEGPLRAQDLRESYIQLVQGVQEWQDGCMYQGEFGLNMKLGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSSFTGTFYLSHREGYGTMYMKTRLFQTHCHNDIVNLLLDCGADVNKCSDEGLTALSMCFLLHYPAQSFKPNVAERTIPEPQEPPKFPVVPILSSSFMDTNLESLYYEVNVPSQGSYELRPPPAPLLLPRVSGSHEGGHFQDTGQCGGSIDHRSSSLKGDSPLVKGSLGHVESGLEDVLGNTDRGSLCSAETKFESNVCVCDFSIELSQAMLERSAQSHSLLKMASPSPCTSSFDKGTMRRMALSMIERRKRWRTIKLLLRRGADPNLCCVPMQVLFLAVKAGDVDGVRLLLEHGARTDICFPPQLSTLTPLHIAAALPGEEGVQIVELLLHAITDVDAKASDEDDTYKPGKLDLLPSSLKLSNEPGPPQAYYSTDTALPEEGGRTALHMACEREDDNKIDRLISHGADILKPVMLRQGEKEAVGTAVDYGYFRFFQDRRIARCPFHTLMPAERETFLARKRLLEYMGLQLRQAVFAKESQWDPTWLYLCKRAELIPSHRMKKKGPSLPRGLDVKEQGQIPFFKFCYQCGRSIGVRLLPCPRCYGILTCSKYCKTKAWTEFHKKDCGDLVAIVTQLEQVSRRREEFQ |
| 分子量 | 106.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANKMY1的3篇代表性文献示例(注：文献为模拟示例，实际引用时需核实):
1. **"ANKMY1 regulates DNA damage response in glioblastoma cells"**
作者：Wang et al. (2018)
摘要：研究发现ANKMY1通过结合HSP70蛋白调控胶质母细胞瘤细胞的DNA修复能力，其表达缺失导致对电离辐射敏感性增加，提示其在癌症治疗中的潜在靶点作用。
2. **"ANKMY1 modulates NF-κB signaling through interaction with IKK complexes"**
作者：Smith & Kumar (2015)
摘要：揭示ANKMY1通过抑制IKK激酶复合物的磷酸化负调控NF-κB信号通路，影响炎症反应和肿瘤细胞存活，为炎症性疾病机制提供新视角。
3. **"Mutations in ANKMY1 linked to neurodevelopmental disorders"**
作者：Hsu et al. (2012)
摘要：首次报道ANKMY1基因罕见突变与自闭症谱系障碍及智力发育迟缓相关，动物模型显示其缺失导致神经元迁移异常和突触功能紊乱。
4. **"Structural insights into ANKMY1-HSP70 complex formation"**
作者：Patel et al. (2020)
摘要：通过X射线晶体学解析ANKMY1的锚蛋白重复结构域与HSP70的互作界面，阐明其分子伴侣功能的结构基础，助力小分子抑制剂开发。
ANKMY1 (Ankyrin Repeat and MYND Domain-Containing Protein 1) is a conserved eukaryotic protein characterized by its unique structural domains, which play critical roles in cellular regulation. The N-terminal region contains multiple ankyrin repeats, known for mediating protein-protein interactions, while the C-terminus harbors a MYND (Myeloid-Nervy-DEAF1) domain, a zinc-binding motif implicated in transcriptional regulation and chromatin remodeling. This dual architecture enables ANKMY1 to act as a molecular scaffold, bridging interactions between chromatin modifiers, transcription factors, and other regulatory complexes.
Functionally, ANKMY1 is linked to neurodevelopment, cell cycle regulation, and DNA damage response. Studies suggest its involvement in neuronal differentiation and migration through modulation of Notch and Wnt signaling pathways. It also interacts with HDAC complexes, influencing gene silencing and chromatin compaction. Additionally, ANKMY1 localizes to sites of DNA damage, aiding repair processes by recruiting repair machinery or stabilizing genomic integrity. Dysregulation of ANKMY1 has been associated with neurodevelopmental disorders and cancer, where aberrant expression may disrupt normal proliferation or differentiation pathways. Despite its emerging significance, mechanistic insights into its precise roles remain limited, prompting ongoing research to elucidate its tissue-specific functions and therapeutic potential. Conservation across species underscores its fundamental biological importance, though organism-specific adaptations may exist. Further studies are needed to map its interactome and define regulatory networks governing its activity.
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