| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANKRD21 |
| Uniprot No | Q86YR6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-584aa |
| 氨基酸序列 | MVAEVCSMPT ASTVKKPFDL RSKMGKWCHH RFPCCRGSGK SNMGTSGDHD DSFMKMLRSK MGKCCRHCFP CCRGSGTSNV GTSGDHENSF MKMLRSKMGK WCCHCFPCCR GSGKSNVGAW GDYDHSAFME PRYHIRREDL DKLHRAAWWG KVPRKDLIVM LRDTDMNKRD KEKRTALHLA SANGNSEVVQ LLLDRRCQLN VLDNKKRTAL IKAIQCQEDE CVLMLLEHGA DRNIPDEYGN TALHYAIYNE DKLMAKALLL YGADIESKNK CGLTPLLLGV HEQKQQVVKF LIKKKANLNV LDRYGRTALI LAVCCGSASI VNLLLEQNVD VSSQDLSGQT AREYAVSSHH HVICELLSDY KEKQMLKISS ENSNPEQDLK LTSEEESQRL KVSENSQPEK MSQEPEINKD CDREVEEEIK KHGSNPVGLP ENLTNGASAG NGDDGLIPQR RSRKPENQQF PDTENEEYHS DEQNDTRKQL SEEQNTGISQ DEILTNKQKQ IEVAEQKMNS ELSLSHKKEE DLLRENSVLQ EEIAMLRLEL DETKHQNQLR ENKILEEIES VKEKTDKLLR AMQLNEEALT KTNI |
| 分子量 | 66.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人ANKRD21蛋白的模拟参考文献(注：文献信息为假设示例，实际研究需根据真实文献调整)：
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1. **文献名称**：*ANKRD21 regulates mitotic progression through kinetochore interaction*
**作者**：Lee, S. et al. (2020)
**摘要**：本研究通过重组表达人ANKRD21蛋白，证实其通过结合纺锤体检查点蛋白调控有丝分裂进程。实验表明ANKRD21缺失会导致染色体排列异常，提示其在维持基因组稳定性中的作用。
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2. **文献名称**：*Structural insights into the ankyrin-repeat domain of ANKRD21*
**作者**：Zhang, Y. et al. (2019)
**摘要**：利用大肠杆菌系统重组表达并纯化ANKRD21蛋白的锚蛋白重复结构域，通过X射线晶体学解析其三维结构，揭示其与泛素化酶相互作用的潜在分子机制。
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3. **文献名称**：*ANKRD21 modulates NF-κB signaling in inflammatory responses*
**作者**：Wang, Q. et al. (2021)
**摘要**：在HEK293细胞中重组表达ANKRD21蛋白，发现其通过抑制IκBα降解负调控NF-κB信号通路，提示其可能作为炎症性疾病的新型治疗靶点。
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如需真实文献，建议检索PubMed或Google Scholar，使用关键词“recombinant ANKRD21”或“ANKRD21 protein function”。
Ankyrin repeat domain-containing protein 21 (ANKRD21) is a conserved eukaryotic protein belonging to the ankyrin repeat family, characterized by tandem repeats of 33-amino-acid motifs that mediate protein-protein interactions. Predominantly localized in the nucleus and cytoplasm, ANKRD21 is implicated in regulating ubiquitination processes by interacting with components of E3 ubiquitin ligase complexes, potentially influencing protein degradation, DNA repair, and cell cycle progression. Studies suggest its involvement in maintaining genomic stability and modulating stress responses, though its precise mechanisms remain under investigation.
ANKRD21 is ubiquitously expressed across tissues, with elevated levels observed in reproductive organs, indicating potential roles in gametogenesis or early development. Dysregulation of ANKRD21 has been tentatively linked to cancer, as aberrant expression correlates with tumor proliferation and metastasis in certain models. However, its exact pathological contributions are not fully defined.
As a recombinant protein, ANKRD21 serves as a valuable tool for probing ubiquitination pathways and cellular stress signaling. Its recombinant form enables in vitro studies on substrate recognition, enzyme complex assembly, and interaction networks, offering insights into therapeutic targeting of ubiquitin-related disorders. Ongoing research aims to clarify its context-dependent functions and therapeutic relevance in human diseases.
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