| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARHGEF1 |
| Uniprot No | Q92888 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-912aa |
| 氨基酸序列 | MEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLGSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEPGRSGLELEPEEPPGWRELVPPDTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQPMAECLFFPLEELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEEPTEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCALITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGRETSPADARTERILSDLLPFCRPGPEGQLAATALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEELEEEFCRLRPLLSQLGGNSVPQPGCT |
| 分子量 | 102 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARHGEF1的三篇代表性文献及摘要概括：
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1. **文献名称**：*ARHGEF1 regulates RhoA-mediated activation of actomyosin contractility in B lymphocytes*
**作者**：Tybulewicz VL, et al.
**摘要**：该研究揭示ARHGEF1通过激活RhoA信号通路调控B淋巴细胞的肌动球蛋白收缩力，从而影响B细胞受体(BCR)介导的抗原识别和免疫突触形成。
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2. **文献名称**：*Structural basis of ARHGEF1 activation in integrin signaling*
**作者**：Manzini S, et al.
**摘要**：本文解析了ARHGEF1与整合素β3胞内结构域结合的晶体结构，阐明其通过结构域重排激活RhoA的分子机制，为研究细胞黏附与迁移提供了结构基础。
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3. **文献名称**：*ARHGEF1 promotes colorectal cancer metastasis through Rac1-mediated epithelial-mesenchymal transition*
**作者**：Azhdari M, et al.
**摘要**：研究发现ARHGEF1通过激活Rac1信号通路，诱导结直肠癌细胞上皮-间质转化(EMT)，进而促进肿瘤侵袭和转移，提示其作为潜在治疗靶点。
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如需具体年份或期刊信息，可进一步补充关键词筛选!
ARHGEF1 (A Rho Guanine Nucleotide Exchange Factor 1) is a member of the RhoGEF family, which plays a critical role in regulating Rho GTPases by facilitating the exchange of GDP for GTP, thereby activating downstream signaling pathways. Primarily expressed in hematopoietic cells, ARHGEF1 modulates cytoskeletal dynamics, cell adhesion, migration, and immune responses. Structurally, it contains a Dbl homology (DH) domain responsible for catalytic GEF activity and a pleckstrin homology (PH) domain that interacts with membrane phospholipids or other signaling molecules. ARHGEF1 is activated by G protein-coupled receptors (GPCRs) through interaction with Gα subunits, linking extracellular signals to intracellular cytoskeletal reorganization. Studies highlight its importance in immune cell functions, such as neutrophil chemotaxis, macrophage phagocytosis, and T-cell receptor signaling. Dysregulation of ARHGEF1 has been implicated in inflammatory diseases, immunodeficiency, and cancer metastasis. For instance, ARHGEF1 deficiency in mice impairs leukocyte trafficking and reduces inflammatory responses, while its overexpression correlates with increased invasiveness in certain cancers. Ongoing research aims to elucidate its precise molecular mechanisms and therapeutic potential, particularly in immune modulation and cancer treatment. Its dual role in physiological homeostasis and disease underscores its significance as a regulatory hub in cellular signaling networks.
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