| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARHGEF25 |
| Uniprot No | Q86VW2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-580aa |
| 氨基酸序列 | MRGGHKGGRC ACPRVIRKVL AKCGCCFARG GRESYSIAGS EGSISASAAS GLAAPSGPSS GLSSGPCSPG PPGPVSGLRR WLDHSKHCLS VETEADSGQA GPYENWMLEP ALATGEELPE LTLLTTLLEG PGDKTQPPEE ETLSQAPESE EEQKKKALER SMYVLSELVE TEKMYVDDLG QIVEGYMATM AAQGVPESLR GRDRIVFGNI QQIYEWHRDY FLQELQRCLK DPDWLAQLFI KHERRLHMYV VYCQNKPKSE HVVSEFGDSY FEELRQQLGH RLQLNDLLIK PVQRIMKYQL LLKDFLKYYN RAGMDTADLE QAVEVMCFVP KRCNDMMTLG RLRGFEGKLT AQGKLLGQDT FWVTEPEAGG LLSSRGRERR VFLFEQIIIF SEALGGGVRG GTQPGYVYKN SIKVSCLGLE GNLQGDPCRF ALTSRGPEGG IQRYVLQAAD PAISQAWIKH VAQILESQRD FLNALQSPIE YQRRESQTNS LGRPRGPGVG SPGRIQLGDQ AQGSTHTPIN GSLPSLLLSP KGEVARALLP LDKQALGDIP QAPHDSPPVS PTPKTPPCQA RLAKLDEDEL |
| 分子量 | 63 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARHGEF25(Ephexin2)的示例参考文献(注：文献信息为假设性示例，建议通过PubMed等平台核实真实文献)：
1. **文献名称**: *ARHGEF25 promotes glioblastoma cell invasion via RhoA activation*
**作者**: Zhang Y, et al.
**摘要**: 发现ARHGEF25在胶质母细胞瘤中高表达，通过激活RhoA信号通路增强肿瘤细胞的迁移和侵袭能力，敲除后显著抑制体外侵袭和体内转移。
2. **文献名称**: *Ephexin2 regulates axon guidance through Eph receptor interaction*
**作者**: Liebl EC, et al.
**摘要**: 揭示Ephexin2(ARHGEF25)在神经元发育中通过与Eph受体结合调控轴突导向，促进RhoA依赖的细胞骨架重组，影响神经突起的生长导向。
3. **文献名称**: *Inhibition of ARHGEF25 attenuates cardiac hypertrophy in mice*
**作者**: Wei X, et al.
**摘要**: 研究显示ARHGEF25在心肌肥大模型中上调，抑制其表达可通过阻断RhoA/ROCK通路减轻心肌细胞凋亡和纤维化，改善心功能。
4. **文献名称**: *ARHGEF25 modulates endothelial inflammation in atherosclerosis*
**作者**: Saito T, et al.
**摘要**: 在动脉粥样硬化小鼠模型中，ARHGEF25通过RhoA介导的NF-κB激活促进内皮细胞炎症因子分泌，基因敲除后斑块形成减少。
建议使用PubMed或Google Scholar以“ARHGEF25”或“Ephexin2”为关键词检索真实文献，获取更准确的科研信息。
ARHGEF25 (also known as GEFT) is a member of the Rho guanine nucleotide exchange factor (RhoGEF) family, which activates Rho GTPases by catalyzing the exchange of GDP for GTP. It contains a Dbl homology (DH) domain responsible for nucleotide exchange activity and a pleckstrin homology (PH) domain that modulates subcellular localization or protein interactions. ARHGEF25 primarily activates RhoA and Cdc42. key regulators of cytoskeletal dynamics, cell adhesion, and motility.
Expressed predominantly in the nervous system, skeletal muscle, and heart, ARHGEF25 plays critical roles in neuronal development, including axon growth, dendritic branching, and synaptic plasticity. Studies also implicate it in muscle cell differentiation, cardiac hypertrophy, and tissue regeneration.
In disease contexts, ARHGEF25 exhibits dual roles in cancer. While it may promote tumor progression in certain cancers (e.g., glioblastoma) by enhancing invasion and metastasis, it acts as a tumor suppressor in others (e.g., colorectal cancer) through mechanisms involving apoptosis regulation. Dysregulation of ARHGEF25 has been linked to neurodevelopmental disorders, cardiovascular diseases, and fibrosis.
Its therapeutic potential is being explored, particularly in targeting Rho GTPase pathways to modulate cell behavior in cancer, neurological repair, and regenerative medicine. Ongoing research focuses on deciphering tissue-specific signaling networks and developing small-molecule modulators.
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