| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASK |
| Uniprot No | Q99683 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1374aa |
| 氨基酸序列 | MSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT |
| 分子量 | 154 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ASK蛋白(以ASK1为例)的3篇模拟参考文献示例，供参考：
1. **文献名称**：重组人ASK1蛋白的表达纯化及其在神经细胞凋亡中的作用
**作者**：Zhang L, et al.
**摘要**：通过大肠杆菌系统成功表达并纯化重组人ASK1蛋白，验证了其激酶活性，证明其在氧化应激诱导的神经细胞凋亡中通过激活p38 MAPK信号通路发挥作用。
2. **文献名称**：基于哺乳动物细胞的重组人ASK1蛋白制备及其药物筛选应用
**作者**：Wang Y, et al.
**摘要**：利用HEK293细胞表达重组人ASK1蛋白，建立了高灵敏度体外激酶活性检测体系，筛选出小分子抑制剂X-23.证实其可通过抑制ASK1-JNK通路缓解肝纤维化模型小鼠的病理进展。
3. **文献名称**：重组人ASK1蛋白结构解析及其与Thioredoxin相互作用研究
**作者**：Tanaka M, et al.
**摘要**：通过冷冻电镜解析了重组人ASK1蛋白的三维结构，揭示了Thioredoxin结合ASK1的分子机制，为开发靶向ASK1的抗氧化疗法提供了结构基础。
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**注**：以上文献内容为示例性质，实际引用请以真实发表的论文为准。建议通过PubMed、Web of Science等数据库以"recombinant human ASK1 protein"为关键词检索相关文献。
**Background of Recombinant Human ASK1 Protein**
Recombinant human apoptosis signal-regulating kinase 1 (ASK1), also known as MAP3K5. is a serine/threonine protein kinase critical in regulating cellular stress responses and apoptosis. ASK1 belongs to the mitogen-activated protein kinase kinase kinase (MAP3K) family and acts as a pivotal mediator in oxidative, endoplasmic reticulum (ER), and cytokine-induced stress signaling. Upon activation by stressors like ROS, TNF-α, or calcium imbalance, ASK1 triggers downstream pathways, including JNK and p38 MAPK cascades, promoting inflammation, apoptosis, or differentiation. Its dysregulation is implicated in diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions.
Produced via recombinant DNA technology in *E. coli* or mammalian expression systems, recombinant ASK1 retains bioactivity for *in vitro* studies. Purification methods (e.g., affinity chromatography) ensure high specificity. Researchers utilize this protein to explore ASK1's role in signaling networks, screen inhibitors for therapeutic development, and model pathological mechanisms. Notably, ASK1 inhibitors are under investigation for treating conditions like non-alcoholic steatohepatitis (NASH), highlighting its translational relevance. This tool has advanced understanding of stress-responsive pathways and their therapeutic targeting.
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