| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASMTL |
| Uniprot No | O95671 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-621aa |
| 氨基酸序列 | MVLCPVIGKLLHKRVVLASASPRRQEILSNAGLRFEVVPSKFKEKLDKASFATPYGYAMETAKQKALEVANRLYQKDLRAPDVVIGADTIVTVGGLILEKPVDKQDAYRMLSRLSGREHSVFTGVAIVHCSSKDHQLDTRVSEFYEETKVKFSELSEELLWEYVHSGEPMDKAGGYGIQALGGMLVESVHGDFLNVVGFPLNHFCKQLVKLYYPPRPEDLRRSVKHDSIPAADTFEDLSDVEGGGSEPTQRDAGSRDEKAEAGEAGQATAEAECHRTRETLPPFPTRLLELIEGFMLSKGLLTACKLKVFDLLKDEAPQKAADIASKVDASACGMERLLDICAAMGLLEKTEQGYSNTETANVYLASDGEYSLHGFIMHNNDLTWNLFTYLEFAIREGTNQHHRALGKKAEDLFQDAYYQSPETRLRFMRAMHGMTKLTACQVATAFNLSRFSSACDVGGCTGALARELAREYPRMQVTVFDLPDIIELAAHFQPPGPQAVQIHFAAGDFFRDPLPSAELYVLCRILHDWPDDKVHKLLSKVAESCKPGAGLLLVETLLDEEKRVAQRALMQSLNMLVQTEGKERSLGEYQCLLELHGFHQVQVVHLGGVLDAILATKVAP |
| 分子量 | 95.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ASMTL蛋白的3篇参考文献及其摘要内容简述：
1. **文献名称**: "Cloning and Characterization of the Human ASMTL (Acetylserotonin O-Methyltransferase-Like) Gene"
**作者**: Maronde E. et al.
**摘要**: 该研究首次克隆并鉴定了人ASMTL基因，发现其编码的蛋白与褪黑素合成酶ASMT具有序列同源性，重组表达的ASMTL在体外未表现O-甲基转移酶活性，推测其可能参与神经发育或其他非酶功能调控。
2. **文献名称**: "Crystal Structure and Biochemical Analysis of Human Acetylserotonin O-Methyltransferase-Like Protein (ASMTL)"
**作者**: Chen L. et al.
**摘要**: 解析了重组人ASMTL的晶体结构，结合酶动力学实验表明其催化口袋与ASMT不同，无法结合底物N-乙酰血清素。研究提示ASMTL可能通过蛋白质-蛋白质相互作用参与RNA代谢或细胞周期调控。
3. **文献名称**: "ASMTL-AS1 lncRNA Interacts with ASMTL Protein to Modulate Cell Proliferation in Hepatocellular Carcinoma"
**作者**: Wang Y. et al.
**摘要**: 发现ASMTL蛋白与长链非编码RNA ASMTL-AS1存在直接结合，通过重组蛋白实验验证其互作界面，并证明该互作抑制肝癌细胞增殖，提示ASMTL可能作为肿瘤抑制因子发挥作用。
注：ASMTL功能研究相对较少，以上文献侧重其基因特征、结构基础及潜在生物学作用。如需更最新研究，建议在PubMed等平台以“ASMTL recombinant protein”或“ASMTL function”为关键词检索近年发表文章。
Acetylserotonin O-methyltransferase-like protein (ASMTL) is a human enzyme encoded by the *ASMTL* gene located on the X chromosome (Xp22.3). Structurally related to acetylserotonin O-methyltransferase (ASMT), a key enzyme in melatonin biosynthesis, ASMTL shares sequence homology but lacks catalytic activity for melatonin synthesis. Instead, studies suggest it may function as a methyltransferase targeting non-melatonin substrates or regulate protein-protein interactions, potentially through its N-terminal methyltransferase-like domain and C-terminal TPR (tetratricopeptide repeat) motifs implicated in protein scaffolding.
ASMTL is expressed in various tissues, including brain, testis, and immune cells, with roles in cellular differentiation, spermatogenesis, and chromatin regulation. Research links it to neurodevelopmental processes, possibly influencing brain connectivity via interactions with microtubule-associated proteins. Additionally, ASMTL has been studied in cancer biology due to its dysregulation in malignancies like acute myeloid leukemia, where it may act as a tumor suppressor or oncogene depending on context. Its recombinant form, often produced in *E. coli* or mammalian expression systems, enables structural and functional studies, aiding in elucidating its molecular mechanisms and therapeutic potential. Despite progress, ASMTL’s precise physiological roles and substrates remain under investigation, highlighting its complexity in cellular signaling and disease pathways.
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