| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYP2B7P1 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-377aa |
| 氨基酸序列 | MELSVLLFLALLTGLLLLLVQRHPNSHGTLPPGPRPLPLLGNLLQMDRRGLLKSFLRFREKYGDVFTVHLGPRPVVMLCGVEAIREALVDNAEAFSGRGKIVIMDPVYQGYGMLFANGNRWKVLRRFSVTTMRDFGMGKRSVEERIQDEAQCLIEELRKSKGALVDPTFLFHSITANIICSIIFGKRFHYQDQEFLKTLNLFCQSFLLISSISSQLFELFSGFLKYFPGAHRQVYKNLQEINAYIGHSVEKHRETLDPSAPRDLIDTYLLHMEKEKSNPHSEFSHQNLIINTLSLFFAGTETTSTTLRYGFLLMLKYPHVAERVYKEIEQVVGPHRPPALDDRAKMPYTEAVIREIQRFADLLPMGVPHIVTQHTSF |
| 分子量 | 69.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CYP2B7P1蛋白的3篇参考文献示例(注：由于CYP2B7P1是假基因，研究较少，以下内容为模拟文献框架)：
1. **"Pseudogene CYP2B7P1 Expression in Human Hepatocytes: A Recombinant Approach"**
*作者：Zhang, L., et al.*
**摘要**：本研究通过重组技术在人肝癌细胞系中表达了CYP2B7P1蛋白，发现其虽无法催化典型CYP2B6底物，但可能通过竞争性结合调控相关代谢通路。
2. **"Structural Insights into the Pseudogene CYP2B7P1 via Recombinant Protein Modeling"**
*作者：Guengerich, F.P., & Hanna, I.H.*
**摘要**：通过重组表达和分子建模分析CYP2B7P1的结构，揭示其活性位点关键氨基酸缺失，解释了其缺乏酶活性的遗传基础。
3. **"CYP2B7P1: A Potential Regulatory Element in Drug Metabolism"**
*作者：Wang, Y., et al.*
**摘要**：重组表达的CYP2B7P1蛋白在体外未显示代谢活性，但其mRNA表达水平与CYP2B6呈负相关，提示其在表观遗传调控中的作用。
**说明**：CYP2B7P1是公认的假基因，相关功能研究较少。以上文献示例基于类似假基因研究框架，实际需进一步查阅最新数据库(如PubMed)获取真实文献。
Recombinant human CYP2B7P1 protein is derived from the CYP2B7P1 pseudogene, a non-functional member of the cytochrome P450 (CYP) superfamily. Cytochrome P450 enzymes are primarily involved in xenobiotic metabolism, drug detoxification, and biosynthesis of sterols or signaling molecules. The CYP2B7P1 pseudogene, located on chromosome 19q13.2. shares high sequence homology with its functional counterpart CYP2B6 but contains disruptive mutations (e.g., frameshifts, premature stop codons), rendering it non-translatable into a functional enzyme. Despite its pseudogene status, CYP2B7P1 may play regulatory roles in neighboring gene expression or CYP2B6 activity through transcriptional interference or non-coding RNA mechanisms. Recombinant CYP2B7P1 protein is artificially expressed in heterologous systems (e.g., E. coli, insect cells) using synthetic constructs that bypass its inherent defects. This engineered protein enables researchers to investigate hypothetical structural or functional vestiges, potential neofunctionalization, or interactions with other drug-metabolizing enzymes. Studies on recombinant CYP2B7P1 could shed light on pseudogene evolution, pharmacogenomic variations, and interindividual differences in drug responses. Its characterization also contributes to understanding CYP2B subfamily diversity, particularly in contexts like cancer biology, where pseudogene dysregulation has been implicated. However, the biological relevance of recombinant CYP2B7P1 remains speculative, as no endogenous protein product has been confirmed in human tissues.
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