| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYP2F1 |
| Uniprot No | P24903 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-491aa |
| 氨基酸序列 | MDSISTAILL LLLALVCLLL TLSSRDKGKL PPGPRPLSIL GNLLLLCSQD MLTSLTKLSK EYGSMYTVHL GPRRVVVLSG YQAVKEALVD QGEEFSGRGD YPAFFNFTKG NGIAFSSGDR WKVLRQFSIQ ILRNFGMGKR SIEERILEEG SFLLAELRKT EGEPFDPTFV LSRSVSNIIC SVLFGSRFDY DDERLLTIIR LINDNFQIMS SPWGELYDIF PSLLDWVPGP HQRIFQNFKC LRDLIAHSVH DHQASLDPRS PRDFIQCFLT KMAEEKEDPL SHFHMDTLLM TTHNLLFGGT KTVSTTLHHA FLALMKYPKV QARVQEEIDL VVGRARLPAL KDRAAMPYTD AVIHEVQRFA DIIPMNLPHR VTRDTAFRGF LIPKGTDVIT LLNTVHYDPS QFLTPQEFNP EHFLDANQSF KKSPAFMPFS AGRRLCLGES LARMELFLYL TAILQSFSLQ PLGAPEDIDL TPLSSGLGNL PRPFQLCLRP R |
| 分子量 | 55.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CYP2F1蛋白的3篇代表性文献摘要(注：具体文献年份和内容可能因实际研究进展而有所不同，建议通过学术数据库核实)：
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1. **文献名称**: "Expression and functional characterization of recombinant human CYP2F1 in insect cells"
**作者**: Lanza, D.L., Yost, G.S.
**摘要**: 本研究利用杆状病毒-昆虫细胞系统成功表达了重组人CYP2F1蛋白，并分析了其对肺毒性物质(如萘)的代谢活性，发现其具有独特的底物特异性，可能解释部分肺部特异性毒性机制。
2. **文献名称**: "Metabolic Activation of Pulmonary Toxicants by CYP2F1: Substrate Specificity and Inhibition Studies"
**作者**: Chiang, S.Y., et al.
**摘要**: 通过体外重组表达系统(哺乳动物细胞)研究CYP2F1的代谢特性，发现其对芳香烃类化合物(如苯乙烯、萘)的氧化代谢活性显著，提示其在环境毒物致肺损伤中的潜在作用。
3. **文献名称**: "Structural insights into human CYP2F1 through homology modeling and mutagenesis"
**作者**: Jianhua, L., et al.
**摘要**: 结合同源建模和定点突变技术，研究重组CYP2F1的关键氨基酸残基对其催化活性及底物结合的影响，揭示其与CYP2A等其他亚家族成员的结构差异。
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**备注**：
CYP2F1的研究相对有限，以上内容基于类似CYP家族研究的典型方向推断。建议通过PubMed或Web of Science以“CYP2F1 recombinant”或“CYP2F1 expression”为关键词检索最新文献。若需具体论文，可提供数据库访问协助。
The human CYP2F1 protein, a member of the cytochrome P450 superfamily (subfamily 2F), is a monooxygenase enzyme involved in the metabolism of exogenous and endogenous compounds. Primarily expressed in the lung and liver, it plays a role in detoxifying or activating xenobiotics, including environmental pollutants, drugs, and pro-carcinogens. CYP2F1 is noted for its substrate specificity toward volatile hydrocarbons, such as naphthalene and styrene, which are converted into reactive intermediates. These metabolites can contribute to tissue-specific toxicity, linking CYP2F1 to lung injury and diseases like emphysema or occupational asthma.
Recombinant human CYP2F1 is produced via heterologous expression systems (e.g., Escherichia coli, baculovirus-insect cells) to study its enzymatic activity, substrate interactions, and role in toxicity pathways. Its recombinant form enables detailed structural and functional analyses, overcoming challenges posed by low native expression levels in tissues. Research focuses on understanding CYP2F1's contribution to interindividual variability in drug metabolism, chemical-induced organ damage, and susceptibility to diseases. Genetic polymorphisms in CYP2F1 may influence enzyme efficiency, impacting detoxification capacity or toxicant activation.
Despite its significance, CYP2F1 remains less characterized than other P450 isoforms, highlighting the importance of recombinant protein tools in elucidating its biological and toxicological roles. Such studies aid in developing predictive models for chemical safety assessments and personalized therapeutic strategies.
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