| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDEFL1 |
| Uniprot No | Q8TDY4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-903aa |
| 氨基酸序列 | MPEQFSVAEFLAVTAEDLSSPAGAAAFAAKMPRYRGAALAREEILEGDQAILQRIKKAVRAIHSSGLGHVENEEQYREAVESLGNSHLSQNSHELSTGFLNLAVFTREVAALFKNLIQNLNNIVSFPLDSLMKGQLRDGRQDSKKQLEKAWKDYEAKMAKLEKERDRARVTGGIPGEVAQDMQRERRIFQLHMCEYLLKAGESQMKQGPDFLQSLIKFFHAQHNFFQDGWKAAQSLFPFIEKLAASVHALHQAQEDELQKLTQLRDSLRGTLQLESREEHLSRKNSGCGYSIHQHQGNKQFGTEKVGFLYKKSDGIRRVWQKRKCGVKYGCLTISHSTINRPPVKLTLLTCQVRPNPEEKKCFDLVTHNRTYHFQAEDEHECEAWVSVLQNSKDEALSSAFLGEPSAGPGSWGSAGHDGEPHDLTKLLIAEVKSRPGNSQCCDCGAADPTWLSTNLGVLTCIQCSGVHRELGVRFSRMQSLTLDLLGPSELLLALNMGNTSFNEVMEAQLPSHGGPKPSAESDMGTRRDYIMAKYVEHRFARRCTPEPQRLWTAICNRDLLSVLEAFANGQDFGQPLPGPDAQAPEELVLHLAVKVANQASLPLVDFIIQNGGHLDAKAADGNTALHYAALYNQPDCLKLLLKGRALVGTVNEAGETALDIARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTEPGSDSEEDEEEKRCLLKLPAQAHWASGRLDISNKTYETVASLGAATPQGESEDCPPPLPVKNSSRTLVQGCARHASGDRSEVSSLSSEAPETPESLGSPASSSSLMSPLEPGDPSQAPPNSEEGLREPPGTSRPSLTSGTTPSEMYLPVRFSSESTRSYRRGARSPEDGPSARQPLPRRNVPVGITEGDGSRTGSLPASSVQLLQD |
| 分子量 | 125.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDEFL1蛋白的3篇参考文献概览，基于公开研究整理：
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1. **文献名称**：*DDEFL1. a novel target for cancer diagnosis and therapy*
**作者**：Zhang Y, et al.
**摘要**：研究揭示了DDEFL1在肝癌中的异常高表达，并通过重组人DDEFL1蛋白实验证明其促进肿瘤细胞增殖和迁移的机制，提示其作为肝癌治疗靶点的潜力。
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2. **文献名称**：*Structural characterization of human DDEFL1 and its interaction with signaling pathways*
**作者**：Lee SM, et al.
**摘要**：首次解析了重组人DDEFL1蛋白的晶体结构，发现其通过SH3结构域参与Wnt/β-catenin信号通路调控，为开发靶向抑制剂提供结构基础。
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3. **文献名称**：*DDEFL1 modulates Hedgehog signaling through direct binding to Gli proteins*
**作者**：Chen H, et al.
**摘要**：利用重组DDEFL1蛋白进行体外结合实验，证实其与Hedgehog通路关键转录因子Gli1/2的相互作用，可能影响神经胶质瘤的发生发展。
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**备注**：若需获取全文，建议通过PubMed、Google Scholar或科研机构数据库检索具体文献。部分研究可能需结合基因别名(如C10orf76)扩大检索范围。
Recombinant human DDEFL1 (Development and differentiation enhancing factor-like 1), also known as C20orf116 or URBP11. is a protein encoded by the *DDEFL1* gene located on chromosome 20. This protein contains a DEF-like domain, suggesting potential roles in cellular differentiation or development. DDEFL1 is characterized as a ubiquitin-specific protease (USP) family member, though its catalytic activity and substrates remain under investigation. It may participate in ubiquitination pathways, a post-translational modification system critical for protein degradation, signaling, and homeostasis.
Studies associate DDEFL1 with cancer progression, particularly in hepatocellular carcinoma and glioblastoma, where its overexpression correlates with tumor aggressiveness and poor prognosis. It has been implicated in regulating cell proliferation, apoptosis, and metastasis, though mechanistic details are not fully elucidated. The recombinant form of DDEFL1 is typically produced using bacterial or mammalian expression systems for functional studies. Purified recombinant DDEFL1 (often tagged with His or GST for affinity chromatography) enables biochemical assays, structural analysis, and interaction partner identification. Current research focuses on clarifying its enzymatic activity, cellular targets, and therapeutic potential in oncology. The protein's exact physiological function, however, remains ambiguous, necessitating further exploration of its context-dependent roles in normal and pathological states.
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