| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAJA4 |
| Uniprot No | Q8WW22 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-397aa |
| 氨基酸序列 | MVKETQYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEGEKFKLISQAYEVLSDPKKRDVYDQGGEQAIKEGGSGSPSFSSPMDIFDMFFGGGGRMARERRGKNVVHQLSVTLEDLYNGVTKKLALQKNVICEKCEGVGGKKGSVEKCPLCKGRGMQIHIQQIGPGMVQQIQTVCIECKGQGERINPKDRCESCSGAKVIREKKIIEVHVEKGMKDGQKILFHGEGDQEPELEPGDVIIVLDQKDHSVFQRRGHDLIMKMKIQLSEALCGFKKTIKTLDNRILVITSKAGEVIKHGDLRCVRDEGMPIYKAPLEKGILIIQFLVIFPEKHWLSLEKLPQLEALLPPRQKVRITDDMDQVELKEFCPNEQNWRQHREAYEEDEDGPQAGVQCQTA |
| 分子量 | 69.41 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DNAJA4蛋白的3篇参考文献示例，包含文献名称、作者及简要摘要内容：
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1. **文献名称**：*DNAJA4 facilitates cell proliferation in colorectal cancer via suppressing P53 activity*
**作者**：Li X, Wang Y, Cheng S, et al.
**摘要**：研究揭示重组人DNAJA4蛋白通过抑制肿瘤抑制蛋白P53的活性，促进结直肠癌细胞增殖。实验显示，敲低DNAJA4可激活P53通路并诱导细胞周期阻滞。
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2. **文献名称**：*Recombinant DNAJA4 attenuates oxidative stress by modulating mitochondrial function in cardiomyocytes*
**作者**：Zhang H, Chen L, Liu Q.
**摘要**：本文验证了重组DNAJA4蛋白在心肌细胞中对氧化应激的调控作用，发现其通过维持线粒体膜电位并减少ROS累积，保护心肌细胞免受损伤。
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3. **文献名称**：*DNAJA4 interacts with viral capsid proteins to enhance enterovirus replication*
**作者**：Kim J, Park SM, Lee K.
**摘要**：该研究利用重组DNAJA4蛋白证实其与肠道病毒衣壳蛋白结合，促进病毒粒子组装和复制，提示DNAJA4可能成为抗病毒治疗的潜在靶点。
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注：上述文献为示例，实际引用时需以真实发表文章为准。若需具体文献，建议通过PubMed或Web of Science以关键词“recombinant human DNAJA4”检索。
Recombinant human DNAJA4 protein is a member of the Hsp40 (heat shock protein 40) family, also known as DnaJ homologs, which function as molecular chaperones in cellular protein homeostasis. DNAJA4. encoded by the DNAJA4 gene, contains a conserved J-domain that interacts with Hsp70 to regulate ATPase activity, facilitating substrate folding, trafficking, and degradation. Unlike some Hsp40 members, DNAJA4 features a unique domain architecture with a glycine/phenylalanine-rich region and a C-terminal substrate-binding domain, enabling selective recognition of misfolded proteins.
Studies suggest DNAJA4 is involved in stress responses, cancer progression, and neurodegenerative diseases. It localizes to the cytoplasm and mitochondria, with roles in mitigating oxidative stress and apoptosis. Dysregulation of DNAJA4 has been linked to tumorigenesis; it is overexpressed in certain cancers (e.g., breast, liver) but downregulated in others (e.g., colorectal), indicating context-dependent functions. Its recombinant form, produced via bacterial or mammalian expression systems, enables in vitro studies of chaperone mechanisms, protein-protein interactions, and therapeutic target validation. Current research focuses on elucidating its precise biological roles and potential as a diagnostic marker or drug target.
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