| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAJB7 |
| Uniprot No | Q7Z6W7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309aa |
| 氨基酸序列 | MVDYYEVLGL QRYASPEDIK KAYHKVALKW HPDKNPENKE EAERKFKEVA EAYEVLSNDE KRDIYDKYGT EGLNGGGSHF DDECEYGFTF HKPDDVFKEI FHERDPFSFH FFEDSLEDLL NRPGSSYGNR NRDAGYFFST ASEYPIFEKF SSYDTGYTSQ GSLGHEGLTS FSSLAFDNSG MDNYISVTTS DKIVNGRNIN TKKIIESDQE REAEDNGELT FFLVNSVANE EGFAKECSWR TQSFNNYSPN SHSSKHVSQY TFVDNDEGGI SWVTSNRDPP IFSAGVKEGG KRKKKKRKEV QKKSTKRNC |
| 分子量 | 61.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于领域常见研究方向模拟的3-4条参考文献示例(注：以下为虚构文献，真实文献需通过学术数据库检索)：
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1. **文献名称**：*DNAJB7 suppresses tumor metastasis by regulating client protein folding and inhibiting EMT*
**作者**：Li, X., et al.
**摘要**：本研究揭示了重组人DNAJB7蛋白通过调节EGFR等客户蛋白的正确折叠，抑制上皮-间质转化(EMT)，从而降低乳腺癌细胞迁移能力。体外实验表明，DNAJB7敲除导致癌细胞侵袭性增强。
2. **文献名称**：*The role of recombinant DNAJB7 in spermatogenesis and male fertility*
**作者**：Wang, Y., et al.
**摘要**：通过构建睾丸特异性过表达DNAJB7的转基因小鼠，发现该蛋白与热休克蛋白HSP70协同作用，维持精子形成过程中蛋白质稳态。缺失DNAJB7导致精子畸形率显著升高。
3. **文献名称**：*Structural and functional analysis of the human DNAJB7 molecular chaperone*
**作者**：Zhang, R., et al.
**摘要**：利用X射线晶体学解析了重组DNAJB7蛋白的J结构域三维构象，揭示了其与HSP70结合的分子机制。体外实验表明，DNAJB7通过识别错误折叠蛋白的疏水区域发挥解聚功能。
4. **文献名称**：*DNAJB7 mitigates Aβ aggregation in Alzheimer’s disease models*
**作者**：Chen, L., et al.
**摘要**：研究发现，重组DNAJB7蛋白在细胞模型中显著减少β-淀粉样蛋白(Aβ)寡聚体形成，并通过激活自噬通路清除毒性聚集体，提示其作为阿尔茨海默病治疗靶点的潜力。
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**建议检索关键词**：
- 真实文献可尝试在PubMed/Google Scholar搜索 "recombinant human DNAJB7"、"DNAJB7 chaperone function"、"DNAJB7 disease" 等。
- 关注领域顶刊如*Journal of Biological Chemistry*、*Cell Stress and Chaperones*等。
Recombinant human DNAJB7 protein is a genetically engineered variant of the DNAJB7 molecule, a member of the DnaJ/Hsp40 family. These proteins act as co-chaperones, assisting Hsp70 in ATP-dependent processes such as protein folding, disaggregation, and degradation. DNAJB7 is classified as a Type II DnaJ protein, characterized by a conserved J-domain for Hsp70 interaction, a glycine/phenylalanine-rich region, and a substrate-binding domain. It is predominantly expressed in the testis and has been implicated in spermatogenesis, although its precise biological roles remain understudied compared to other DnaJ members.
Interest in recombinant DNAJB7 stems from its potential regulatory functions in cellular stress responses and protein quality control. Its overexpression or dysfunction has been tentatively linked to pathologies, including cancer metastasis and neurodegeneration, though mechanistic insights are limited. Producing recombinant DNAJB7 via bacterial or mammalian expression systems enables large-scale purification for functional studies, overcoming challenges associated with low endogenous expression levels. This facilitates structural analyses, interaction mapping with Hsp70 partners, and exploration of its role in disease models. Current research focuses on deciphering its client proteins, stress-responsive behavior, and therapeutic potential as a biomarker or target for diseases involving proteostatic imbalance. The recombinant tool also supports high-throughput screening for modulators of chaperone networks.
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