| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAJB14 |
| Uniprot No | Q8TBM8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-379aa |
| 氨基酸序列 | MEGNRDEAEKCVEIAREALNAGNREKAQRFLQKAEKLYPLPSARALLEIIMKNGSTAGNSPHCRKPSGSGDQSKPNCTKDSTSGSGEGGKGYTKDQVDGVLSINKCKNYYEVLGVTKDAGDEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGNEEQACNHQNNGRFNFHRGCEADITPEDLFNIFFGGGFPSGSVHSFSNGRAGYSQQHQHRHSGHEREEERGDGGFSVFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGTGQTIKMQTENLGVVYYVNKDFKNEYKGMLLQKVEKSVEEDYVTNIRNNCWKERQQKTDMQYAAKVYRDDRLRRKADALSMDNCKELERLTSLYKGG |
| 分子量 | 68.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DNAJB14蛋白的示例参考文献(**注：以下文献为示例，实际引用需通过学术数据库查询**)：
1. **文献名称**：*Recombinant Human DNAJB14 Facilitates Neuroprotection in Cellular Stress Models*
**作者**：Smith A, et al.
**摘要**：本研究通过大肠杆菌系统表达重组人DNAJB14蛋白，验证其作为分子伴侣在神经元细胞中减轻错误折叠蛋白聚集的能力，并证明其在氧化应激模型中的保护作用。
2. **文献名称**：*Structural and Functional Analysis of DNAJB14 in Cancer Cell Proliferation*
**作者**：Zhang L, Wang Q.
**摘要**：通过X射线晶体学解析了重组DNAJB14的结构，并发现其J结构域对抑制癌细胞增殖的关键作用，为靶向治疗提供新思路。
3. **文献名称**：*DNAJB14 Interaction with HSP70: Implications for Protein Quality Control*
**作者**：Johnson R, et al.
**摘要**：报道了重组DNAJB14与HSP70伴侣系统的协同机制，揭示了其在维持线粒体蛋白质稳态中的调控功能。
4. **文献名称**：*High-Yield Production of Recombinant DNAJB14 and Its Role in Viral Infection*
**作者**：Lee S, et al.
**摘要**：开发了哺乳动物细胞表达系统高效制备重组DNAJB14的方法，并发现其通过调控宿主抗病毒反应抑制RNA病毒复制。
建议通过**PubMed**或**Google Scholar**输入关键词(如“recombinant DNAJB14” “human DNAJB14 function”)获取真实文献。
Recombinant human DNAJB14 protein is a molecular chaperone belonging to the HSP40/DNAJ family, which functions as a co-chaperone for heat shock protein 70 (HSP70) in regulating protein folding, quality control, and stress response. DNAJB14 is localized primarily in the endoplasmic reticulum (ER) and is implicated in ER-associated degradation (ERAD) and the unfolded protein response (UPR). Structurally, it contains a conserved J-domain that mediates interaction with HSP70. a glycine/phenylalanine-rich (G/F) region, and a C-terminal substrate-binding domain. Unlike some DNAJ proteins, DNAJB14 lacks a canonical cysteine-rich zinc finger domain, suggesting distinct substrate specificity.
Recombinant DNAJB14 is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. It is utilized to investigate mechanisms of protein misfolding diseases, such as neurodegenerative disorders and cancer, where ER stress and proteostasis dysregulation are key contributors. Research highlights its role in regulating tau aggregation, modulating ERAD substrates, and maintaining cellular resilience under stress. Additionally, it serves as a tool for exploring therapeutic strategies targeting chaperone networks. The protein’s recombinant form enables high-purity, scalable production for biochemical assays, structural studies, and drug screening, advancing our understanding of proteostasis mechanisms in health and disease.
×
