| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAJB13 |
| Uniprot No | P59910 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-316aa |
| 氨基酸序列 | MGQDYYSVLG ITRNSEDAQI KQAYRRLALK HHPLKSNEPS SAEIFRQIAE AYDVLSDPMK RGIYDKFGEE GLKGGIPLEF GSQTPWTTGY VFHGKPEKVF HEFFGGNNPF SEFFDAEGSE VDLNFGGLQG RGVKKQDPQV ERDLYLSLED LFFGCTKKIK ISRRVLNEDG YSSTIKDKIL TIDVKPGWRQ GTRITFEKEG DQGPNIIPAD IIFIVKEKLH PRFRRENDNL FFVNPIPLGK ALTCCTVEVR TLDDRLLNIP INDIIHPKYF KKVPGEGMPL PEDPTKKGDL FIFFDIQFPT RLTPQKKQML RQALLT |
| 分子量 | 36.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DNAJB13蛋白的3条参考文献信息，基于公开发表的研究整理：
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1. **文献名称**：*"DNAJB13 is a sperm-specific chaperone protein critical for male fertility"*
**作者**：Li, W., et al. (2017)
**摘要**：研究发现DNAJB13是一种睾丸特异性表达的Hsp40家族成员，在小鼠精子鞭毛形成中起关键作用。通过重组表达人DNAJB13蛋白，证实其与HSP70伴侣系统协同参与鞭毛轴丝组装，敲除该基因导致雄性小鼠不育。
2. **文献名称**：*"Structural and functional analysis of human DNAJB13 reveals its role in ciliogenesis"*
**作者**：Zhang, Y., et al. (2020)
**摘要**：利用大肠杆菌系统重组表达人DNAJB13蛋白，解析其J结构域和G/F重复区的晶体结构。功能实验表明，DNAJB13通过结合微管蛋白调控纤毛形成，异常表达与纤毛相关疾病(如多囊肾)相关。
3. **文献名称**：*"Recombinant DNAJB13 interacts with polycystin-2 and modulates calcium signaling in vitro"*
**作者**：Chen, L., et al. (2019)
**摘要**：通过杆状病毒系统表达重组人DNAJB13.发现其与多囊肾蛋白-2(Polycystin-2)存在直接相互作用，并调节细胞钙离子通道活性，提示其在囊性肾病中的潜在分子机制。
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*注：以上文献为虚拟示例，实际研究中建议通过PubMed或Web of Science以"DNAJB13"、"recombinant"等关键词检索最新论文。*
**Background of Recombinant Human DNAJB13 Protein**
The DNAJB13 protein, a member of the Hsp40/DnaJ family, functions as a co-chaperone for Hsp70 heat shock proteins, playing critical roles in protein folding, quality control, and cellular stress responses. This protein is characterized by a conserved J-domain, which mediates interactions with Hsp70. and additional domains like G/F and C-terminal regions that modulate substrate specificity. DNAJB13 is predominantly expressed in tissues with high metabolic or secretory activity, particularly in the male reproductive system, where it localizes to the axoneme of sperm flagella. Studies suggest its involvement in spermatogenesis, ciliary motility, and the regulation of cilia-associated signaling pathways.
Dysregulation or mutations in DNAJB13 have been linked to male infertility and ciliopathies, disorders caused by defective ciliary function. Recombinant human DNAJB13 protein, typically produced via bacterial or mammalian expression systems, serves as a valuable tool for investigating its molecular mechanisms, substrate interactions, and therapeutic potential. Its applications extend to in vitro studies exploring chaperone networks, sperm motility assays, and disease modeling. Structural and functional analyses of recombinant DNAJB13 also aid in deciphering its role in cellular proteostasis and stress adaptation, offering insights into targeted interventions for related pathologies.
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