| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAJB9 |
| Uniprot No | Q9UBS3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-223aa |
| 氨基酸序列 | MATPQSIFIFAICILMITELILASKSYYDILGVPKSASERQIKKAFHKLAMKYHPDKNKSPDAEAKFREIAEAYETLSDANRRKEYDTLGHSAFTSGKGQRGSGSSFEQSFNFNFDDLFKDFGFFGQNQNTGSKKRFENHFQTRQDGGSSRQRHHFQEFSFGGGLFDDMFEDMEKMFSFSGFDSTNQHTVQTENRFHGSSKHCRTVTQRRGNMVTTYTDCSGQ |
| 分子量 | 51.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DNAJB9蛋白的文献示例(文献信息为虚构，仅供示例参考)：
1. **文献名称**：*DNAJB9 as a Biomarker in Fibrillary Glomerulonephritis*
**作者**：Smith A, et al.
**摘要**：该研究证明重组人DNAJB9蛋白在纤维性肾小球肾炎(FGN)患者肾脏组织中特异性高表达，提出其可作为区分FGN与其他肾病的生物标志物，并揭示了其与错误折叠蛋白清除的关联。
2. **文献名称**：*Recombinant DNAJB9 Facilitates Tau Protein Folding in Neurodegenerative Models*
**作者**：Chen L, et al.
**摘要**：通过体外表达重组人DNAJB9蛋白，研究证实其作为分子伴侣与Tau蛋白相互作用，减少错误折叠聚集，为阿尔茨海默病中蛋白毒性应激的调控机制提供了新见解。
3. **文献名称**：*Structural Insights into DNAJB9 Chaperone Function*
**作者**：Wang X, et al.
**摘要**：利用重组DNAJB9蛋白进行晶体结构分析，揭示了其J结构域与HSP70协同作用的关键位点，阐明其通过调控ATP酶活性辅助蛋白折叠的分子机制。
4. **文献名称**：*DNAJB9 Knockdown Aggravates ER Stress in Hepatocellular Carcinoma*
**作者**：Kim Y, et al.
**摘要**：实验发现重组DNAJB9蛋白可缓解肝癌细胞的内质网应激(ERS)，抑制细胞凋亡，提示其在肿瘤进展中的双重调控作用可能与应激信号通路有关。
(注：以上文献为模拟示例，实际引用需以真实发表的论文为准。)
Recombinant human DNAJB9 is a molecular chaperone protein belonging to the HSP40 (Heat Shock Protein 40) family, also known as J-domain proteins. It plays a critical role in protein quality control by regulating the ATPase activity of HSP70 chaperones, aiding in folding nascent polypeptides, resolving misfolded protein aggregates, and directing damaged proteins for degradation. DNAJB9 is primarily localized in the endoplasmic reticulum (ER), where it participates in the unfolded protein response (UPR) to mitigate ER stress. Its structure includes a conserved J-domain that interacts with HSP70 and substrate-binding regions to recognize client proteins.
Recombinant DNAJB9 is produced using expression systems like *E. coli* or mammalian cells for biochemical and biomedical studies. It has gained attention for its association with fibrogenic diseases, particularly as a biomarker in fibrinogen Aα-chain amyloidosis and other fibrotic kidney disorders. Research focuses on its dual roles in cytoprotection and disease pathology, particularly under cellular stress conditions. Studies also explore its potential therapeutic applications, such as modulating protein aggregation in neurodegenerative diseases or cancer. The recombinant protein serves as a tool to dissect chaperone networks and develop targeted therapies for protein-misfolding disorders.
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