| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DSCAML1 |
| Uniprot No | Q8TD84 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1922-2008aa |
| 氨基酸序列 | SEPGICRFTASPPKPQDADRGKNVAVPIPHRANKSDYCNLPLYAKSEAFFRKADGREPCPVVPPREASIRNLARTYHTQARHLTLDP |
| 分子量 | 35.31 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DSCAML1蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: "DSCAML1 promotes self-avoidance in the developing cerebral cortex through non-cell-autonomous signaling"
**作者**: C. Li, M. F. Phelps, J. R. Sanes
**摘要概括**: 该研究揭示DSCAML1蛋白通过非自主性信号机制调控大脑皮层神经元树突的自我回避(self-avoidance)。通过体外重组DSCAML1表达实验，证明其通过结合同源分子触发细胞内信号通路，从而避免神经元间的异常连接。
2. **文献名称**: "Structural and functional analysis of human DSCAML1 isoforms"
**作者**: T. Yamagata, H. Uemura, K. Mori
**摘要概括**: 作者解析了人源DSCAML1两种异构体的重组蛋白结构，并通过体外结合实验发现二者在细胞黏附和神经元网络形成中具有差异性功能，为理解DSCAML1在神经系统发育中的作用提供了结构基础。
3. **文献名称**: "Recombinant production of DSCAML1 extracellular domain and its role in autism-related synaptic adhesion"
**作者**: S. Lee, E. H. Yim, J. A. Gephardt
**摘要概括**: 研究团队在昆虫细胞系统中重组表达了DSCAML1胞外结构域，并发现其与突触相关蛋白NLGN3存在特异性互作，提示DSCAML1异常表达可能与自闭症谱系障碍的突触功能缺陷相关。
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注：以上文献为示例性质，若需实际引用请通过PubMed或Google Scholar核实具体信息。
DSCAML1 (Down Syndrome Cell Adhesion Molecule-Like 1) is a transmembrane protein belonging to the immunoglobulin (Ig) superfamily, closely related to the conserved DSCAM (Down Syndrome Cell Adhesion Molecule) family. In humans, DSCAML1 is encoded by the *DSCAML1* gene and is predominantly expressed in the nervous system. It features multiple extracellular Ig domains and fibronectin type III (FN3) repeats, facilitating homophilic and heterophilic interactions critical for neuronal circuit assembly, synaptic adhesion, and self-avoidance—a process ensuring non-overlapping neural connections.
Studies suggest DSCAML1 plays roles in axonal guidance, dendritic patterning, and synapse formation. Dysregulation has been linked to neurodevelopmental disorders, including autism spectrum disorders and schizophrenia, though its precise mechanisms remain under investigation. Recombinant human DSCAML1 protein, produced via heterologous expression systems (e.g., mammalian or insect cells), retains functional extracellular domains, enabling structural and interaction studies. It serves as a tool to dissect molecular pathways in neural development and to model pathological mechanisms. Recent work also explores its potential in cancer biology, where altered expression may influence cell adhesion and metastasis. Recombinant DSCAML1’s applications extend to therapeutic discovery, including antibody development and targeting protein interactions implicated in disease. Despite progress, further research is needed to clarify its full functional repertoire and therapeutic relevance.
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