| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DTNA |
| Uniprot No | Q9Y4J8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-743aa |
| 氨基酸序列 | MIEDSGKRGN TMAERRQLFA EMRAQDLDRI RLSTYRTACK LRFVQKKCNL HLVDIWNVIE ALRENALNNL DPNTELNVSR LEAVLSTIFY QLNKRMPTTH QIHVEQSISL LLNFLLAAFD PEGHGKISVF AVKMALATLC GGKIMDKLRY IFSMISDSSG VMVYGRYDQF LREVLKLPTA VFEGPSFGYT EQSARSCFSQ QKKVTLNGFL DTLMSDPPPQ CLVWLPLLHR LANVENVFHP VECSYCHSES MMGFRYRCQQ CHNYQLCQDC FWRGHAGGSH SNQHQMKEYT SWKSPAKKLT NALSKSLSCA SSREPLHPMF PDQPEKPLNL AHIVDTWPPR PVTSMNDTLF SHSVPSSGSP FITRSSPPKD SEVEQNKLLA RAAPAFLKGK GIQYSLNVAD RLADEHVLIG LYVNMLRNNP SCMLESSNRL DEEHRLIARY AARLAAESSS SQPPQQRSAP DISFTIDANK QQRQLIAELE NKNREILQEI QRLRLEHEQA SQPTPEKAQQ NPTLLAELRL LRQRKDELEQ RMSALQESRR ELMVQLEGLM KLLKTQGAGS PRSSPSHTIS RPIPMPIRSA SACSTPTHTP QDSLTGVGGD VQEAFAQSSR RNLRNDLLVA ADSITNTMSS LVKELNSEVG SETESNVDSE FARTQFEDLV PSPTSEKAFL AQIHARKPGY IHSGATTSTM RGDMVTEDAD PYVQPEDENY ENDSVRQLEN ELQMEEYLKQ KLQDEAYQVS LQG |
| 分子量 | 83.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DTNA蛋白的3篇模拟参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**: *Expression and Functional Characterization of Recombinant Human α-Dystrobrevin (DTNA) in Muscle Cells*
**作者**: Smith A, et al.
**摘要**: 本研究成功表达并纯化了重组人DTNA蛋白，证实其通过与dystrophin蛋白复合物相互作用维持肌细胞膜稳定性，为研究DTNA在肌肉疾病中的作用提供了工具。
2. **文献名称**: *DTNA Recombinant Protein Improves Cardiac Function in a Duchenne Muscular Dystrophy Model*
**作者**: Chen L, et al.
**摘要**: 利用重组DTNA蛋白治疗缺乏dystrophin的小鼠模型，结果表明其通过修复肌膜完整性改善心脏收缩功能，提示DTNA可能作为潜在治疗分子。
3. **文献名称**: *Structural Analysis of Recombinant DTNA and Its Interaction with Syntrophin*
**作者**: Gonzalez R, et al.
**摘要**: 通过X射线晶体学解析了重组人DTNA蛋白的三维结构，揭示了其与syntrophin蛋白的结合位点，为研究信号转导机制提供了结构基础。
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**说明**：以上文献为示例性质，实际研究中请通过PubMed、Google Scholar等平台检索具体文献(关键词：*recombinant DTNA protein*, *human α-dystrobrevin expression*)。DTNA研究多集中于其在肌肉组织中的功能及与遗传性疾病的关联。
**Recombinant human DTNA protein** is a genetically engineered form of the dystrobrevin alpha (DTNA) protein, a key component of the dystrophin-associated protein complex (DAPC). This complex stabilizes cell membranes, particularly in muscle and neural tissues, by linking the cytoskeleton to the extracellular matrix. DTNA interacts with dystrophin and other DAPC members, playing roles in mechanical stability, signal transduction, and cellular organization. Mutations in DTNA are linked to muscular dystrophy, cardiomyopathies, and neurological disorders, driving interest in its functional study.
Produced via heterologous expression systems (e.g., *E. coli*, mammalian cells*), recombinant DTNA enables *in vitro* studies of its structure-function relationships, binding partners (e.g., syntrophins, dystrophin), and pathological mechanisms. It is purified using affinity tags (e.g., His-tag) and chromatography, ensuring high specificity. Applications include exploring DAPC dysfunction in diseases, drug screening for neuromuscular disorders, and structural biology to map interaction domains. Its recombinant form bypasses challenges in isolating native DTNA from tissues, offering a scalable tool for mechanistic and therapeutic research. Studies using recombinant DTNA have clarified its role in nitric oxide signaling, cytoskeletal organization, and disease pathways, highlighting its potential as a therapeutic target.
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