| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DSCR8 |
| Uniprot No | Q96T75 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-97aa |
| 氨基酸序列 | MKEPGPNFVT VRKGLHSFKM AFVKHLLLFL SPRLECSGSI TDHCSLHLPV QEILMSQPPE QLGLQTNLGN QESSGMMKLF MPRPKVLAQY ESIQFMP |
| 分子量 | 10.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于关于重组人DSCR8蛋白的研究相对较少，且可用文献有限，以下提供**假设性的参考文献示例**(基于对相关领域研究的合理推测)，供参考格式和内容。实际研究中建议通过学术数据库进一步确认最新进展。
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1. **文献名称**：Expression and Purification of Recombinant Human DSCR8 Protein in Escherichia coli
**作者**：Zhang Y et al.
**摘要**：本研究成功构建了人DSCR8基因的原核表达载体，并在大肠杆菌中实现可溶性表达。通过亲和层析和离子交换层析纯化，获得了高纯度的重组蛋白，并通过Western blot验证其免疫活性，为后续功能研究提供材料基础。
2. **文献名称**：Functional Characterization of DSCR8 in Neuronal Development Using Recombinant Protein
**作者**：Lee S et al.
**摘要**：通过体外细胞实验发现，重组人DSCR8蛋白可调节神经突触相关蛋白的表达，并促进神经元分化。研究提示DSCR8可能在唐氏综合征的神经发育异常中发挥作用。
3. **文献名称**：Structural Insights into DSCR8 by X-ray Crystallography and Its RNA-Binding Activity
**作者**：Martinez R et al.
**摘要**：首次解析了重组人DSCR8蛋白的晶体结构，揭示其含有一个保守的RNA结合域。体外结合实验证实DSCR8与特定mRNA序列相互作用，支持其在转录后调控中的潜在功能。
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**备注**：DSCR8作为唐氏综合症关键区域(21q22)中的基因之一，其功能尚不明确，相关重组蛋白的研究较少。以上示例基于典型蛋白研究逻辑构建，实际文献需结合具体研究目标检索(如基因调控、疾病模型等)。
Recombinant human DSCR8 (Down Syndrome Critical Region 8) protein is a genetically engineered form of the DSCR8 protein encoded by the RCAN3 gene located on human chromosome 21. This region is associated with Down syndrome (trisomy 21), as overdosage of genes in this chromosomal segment is linked to developmental and cognitive features of the disorder. DSCR8. a member of the RCAN (Regulator of Calcineurin) family, is implicated in modulating calcium-dependent signaling pathways, particularly through interactions with calcineurin and NFAT (nuclear factor of activated T-cells) transcription factors, which regulate immune responses, cardiac development, and neuronal function. Recombinant DSCR8 is typically expressed in heterologous systems like *E. coli* or mammalian cells to ensure proper post-translational modifications. Its production enables functional studies to elucidate its role in Down syndrome-related pathologies, including neurodevelopmental abnormalities and congenital heart defects. Research also explores its potential involvement in other conditions linked to calcium signaling dysregulation, such as Alzheimer’s disease or cardiac hypertrophy. By providing a standardized, pure protein source, recombinant DSCR8 facilitates biochemical assays, structural studies, and therapeutic target validation, contributing to broader insights into chromosome 21 gene dosage effects and molecular pathways in Down syndrome.
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