| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DSCR2 |
| Uniprot No | O95456 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-288aa |
| 氨基酸序列 | MAATFFGEVVKAPCRAGTEDEEEEEEGRRETPEDREVRLQLARKREVRLLRRQTKTSLEVSLLEKYPCSKFIIAIGNNAVAFLSSFVMNSGVWEEVGCAKLWNEWCRTTDTTHLSSTEAFCVFYHLKSNPSVFLCQCSCYVAEDQQYQWLEKVFGSCPRKNMQITILTCRHVTDYKTSESTGSLPSPFLRALKTQNFKDSACCPLLEQPNIVHDLPAAVLSYCQVWKIPAILYLCYTDVMKLDLITVEAFKPILSTRSLKGLVKNIPQSTEILKKLMTTNEIQSNIYT |
| 分子量 | 57.42 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DSCR2蛋白的参考文献示例(内容为模拟虚构)：
1. **《Expression and Purification of Recombinant Human DSCR2 in Escherichia coli》**
- 作者：J. Smith, L. Zhao
- 摘要：研究报道了通过大肠杆菌系统高效表达重组人DSCR2蛋白的方法，采用His标签亲和层析技术纯化，并验证了其结构完整性，为后续功能研究奠定基础。
2. **《Structural Characterization of DSCR2 Reveals a Novel Calcium-Binding Domain》**
- 作者：M. Tanaka, et al.
- 摘要：通过X射线晶体学解析了重组DSCR2蛋白的三维结构，发现其N端存在新型钙离子结合域，提示该蛋白可能参与钙依赖的细胞信号调控。
3. **《DSCR2 Modulates NFAT Signaling in T-cell Development》**
- 作者：R. Gupta, S. Park
- 摘要：利用重组DSCR2蛋白进行体外功能实验，证明其通过抑制NFAT转录因子入核调控T细胞活化，揭示了DSCR2在免疫应答中的潜在作用。
4. **《Proteomic Analysis of DSCR2-Interacting Proteins in Cancer Metastasis》**
- 作者：C. Wang, et al.
- 摘要：通过免疫共沉淀联合质谱分析，发现重组DSCR2与细胞骨架蛋白相互作用，影响肿瘤细胞迁移能力，提示其作为转移抑制因子的可能性。
Recombinant human DSCR2 (Down Syndrome Critical Region Gene 2) protein is derived from the *DSCR2* gene located on human chromosome 21q22.13. within a region associated with Down syndrome phenotypes. DSCR2 encodes a transmembrane glycoprotein implicated in cellular signaling, adhesion, and immune regulation. The protein’s extracellular domain contains conserved immunoglobulin-like folds, suggesting roles in cell-cell or cell-matrix interactions. Its expression is detected in immune cells (e.g., monocytes, dendritic cells), endothelial cells, and tissues like the spleen and placenta.
Though its precise biological mechanisms remain incompletely characterized, DSCR2 has been linked to inflammatory responses, osteoclast differentiation, and modulation of NF-κB signaling. Studies suggest it interacts with Toll-like receptors (TLRs) to regulate innate immunity. Overexpression in Down syndrome may contribute to immune dysfunction or developmental anomalies. Recombinant DSCR2 is typically produced using mammalian expression systems to ensure proper post-translational modifications. It serves as a vital tool for functional studies, antibody development, and exploring its potential as a therapeutic target for inflammatory diseases or cancers. Current research also focuses on deciphering its role in neurodevelopment and its association with trisomy 21-related pathologies.
×
