| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DSCAM |
| Uniprot No | O60469 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1922-2008aa |
| 氨基酸序列 | SEPGICRFTASPPKPQDADRGKNVAVPIPHRANKSDYCNLPLYAKSEAFFRKADGREPCPVVPPREASIRNLARTYHTQARHLTLDP |
| 分子量 | 35.31 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人DSCAM蛋白相关的模拟参考文献及摘要概括：
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1. **文献名称**：*Structural insights into the homophilic complex of human DSCAM*
**作者**：Chen, X. et al.
**摘要**：本研究解析了重组人DSCAM胞外域的高分辨率晶体结构，揭示了其通过免疫球蛋白结构域介导的同源二聚化机制。实验通过HEK293系统表达并纯化DSCAM蛋白，证明其选择性结合特性对神经突触的精准导向至关重要。
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2. **文献名称**：*Recombinant DSCAM regulates axon guidance via Slit-Robo signaling*
**作者**：Wang, L. & Smith, J.D.
**摘要**：利用昆虫细胞表达系统制备重组人DSCAM蛋白，发现其通过与Slit-Robo通路配体的相互作用调控轴突导向功能。体外实验证实，DSCAM与Robo受体的结合依赖其可变剪接异构体的特定结构域。
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3. **文献名称**：*Functional characterization of DSCAM in Alzheimer’s disease pathology*
**作者**：Garcia, S. et al.
**摘要**：通过重组表达人DSCAM蛋白，研究其在阿尔茨海默病中的潜在作用。结果显示，DSCAM与β-淀粉样蛋白寡聚体的结合可能加剧突触功能障碍，提示其作为治疗靶点的可能性。
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4. **文献名称**：*High-yield purification of human DSCAM for antibody development*
**作者**：Kim, H. et al.
**摘要**：优化重组人DSCAM在CHO细胞中的表达条件，建立亲和层析纯化流程，获得高纯度蛋白用于单克隆抗体制备。该抗体可特异性识别DSCAM在神经细胞表面的表达定位。
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**注**：以上文献信息为模拟示例，实际研究中需查询具体数据库获取真实文献。
Recombinant human DSCAM (Down Syndrome Cell Adhesion Molecule) is a genetically engineered protein derived from the human DSCAM gene, located on chromosome 21. Initially linked to Down syndrome due to its gene dosage effect in trisomy 21. DSCAM belongs to the immunoglobulin superfamily and plays critical roles in neural development, including axon guidance, synaptic formation, and neuronal circuit assembly. It exhibits remarkable diversity through alternative splicing, generating thousands of isoforms that mediate specific cell-cell recognition during brain wiring. Beyond the nervous system, DSCAM is implicated in immune regulation, with studies suggesting roles in dendritic cell function and pathogen response.
The recombinant form, produced via expression systems like mammalian or insect cells, enables precise study of DSCAM’s molecular interactions and signaling pathways. Researchers utilize it to investigate its dual roles in neurodevelopmental disorders and immune dysregulation, particularly in Down syndrome-associated pathologies. Its structural domains, including immunoglobulin-like and fibronectin type III repeats, make it a focus for understanding adhesion mechanisms and designing therapeutic strategies. Recombinant DSCAM also serves as a tool for developing diagnostic assays or targeted therapies for conditions involving disrupted cell adhesion or immune function, highlighting its relevance in both basic research and translational biomedicine.
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