| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DTX3L |
| Uniprot No | Q8TDB6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-740aa |
| 氨基酸序列 | MASHLRPPSPLLVRVYKSGPRVRRKLESYFQSSKSSGGGECTVSTQEHEAPGTFRVEFSERAAKERVLKKGEHQILVDEKPVPIFLVPTENSIKKNTRPQISSLTQSQAETPSGDMHQHEGHIPNAVDSCLQKIFLTVTADLNCNLFSKEQRAYITTLCPSIRKMEGHDGIEKVCGDFQDIERIHQFLSEQFLESEQKQQFSPSMTERKPLSQQERDSCISPSEPETKAEQKSNYFEVPLPYFEYFKYICPDKINSIEKRFGVNIEIQESSPNMVCLDFTSSRSGDLEAARESFASEFQKNTEPLKQECVSLADSKQANKFKQELNHQFTKLLIKEKGGELTLLGTQDDISAAKQKISEAFVKIPVKLFAANYMMNVIEVDSAHYKLLETELLQEISEIEKRYDICSKVSEKGQKTCILFESKDRQVDLSVHAYASFIDAFQHASCQLMREVLLLKSLGKERKHLHQTKFADDFRKRHPNVHFVLNQESMTLTGLPNHLAKAKQYVLKGGGMSSLAGKKLKEGHETPMDIDSDDSKAASPPLKGSVSSEASELDKKEKGICVICMDTISNKKVLPKCKHEFCAPCINKAMSYKPICPTCQTSYGIQKGNQPEGSMVFTVSRDSLPGYESFGTIVITYSMKAGIQTEEHPNPGKRYPGIQRTAYLPDNKEGRKVLKLLYRAFDQKLIFTVGYSRVLGVSDVITWNDIHHKTSRFGGPEMYGYPDPSYLKRVKEELKAKGIE |
| 分子量 | 107.03 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DTX3L蛋白的3篇参考文献及其核心研究内容：
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1. **文献名称**：*PARP9-DTX3L ubiquitin ligase targets host histone H2BJ and viral 3C protease to enhance interferon signaling and control viral infection*
**作者**：Jubin Zhou et al.
**摘要**：该研究揭示了DTX3L与PARP9形成的复合物通过泛素化修饰宿主组蛋白H2BJ和病毒3C蛋白酶，增强干扰素信号通路，从而抑制病毒复制，阐明了DTX3L在抗病毒免疫中的作用机制。
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2. **文献名称**：*DTX3L promotes genome integrity through modulation of CHFR ubiquitylation during DNA damage response*
**作者**：Qiang Wang et al.
**摘要**：研究发现DTX3L作为E3泛素连接酶，通过调控CHFR蛋白的泛素化修饰参与DNA损伤应答，维持基因组稳定性，为DTX3L在癌症发生中的作用提供理论依据。
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3. **文献名称**：*DTX3L interacts with BCR-ABL and regulates its oncogenic activity through ubiquitination*
**作者**：Luis G. Aguiar et al.
**摘要**：该文报道DTX3L通过与BCR-ABL癌蛋白相互作用，介导其泛素化降解，抑制慢性髓系白血病细胞增殖，提示DTX3L在靶向治疗中的潜在应用价值。
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**注意**：若需获取原文，建议通过PubMed或期刊官网查询具体DOI信息。DTX3L的研究多聚焦于其E3泛素连接酶活性、DNA修复及免疫调控功能。
The human DTX3L protein, a member of the Deltex (DTX) family of E3 ubiquitin ligases, plays a critical role in regulating protein degradation and signaling pathways. It contains a conserved N-terminal PDZ domain and a C-terminal RING finger domain, characteristic of E3 ligases involved in substrate recognition and ubiquitination. Unlike other Deltex proteins primarily associated with the Notch pathway, DTX3L exhibits distinct functional roles through interactions with partner proteins like B aggression lymphoma (BAL) and ADP-ribosyltransferase PARP9/ARTD9. This unique DTX3L-PARP9 complex is implicated in DNA damage repair, immune regulation, and inflammatory responses. Studies highlight its involvement in modulating NF-κB signaling, interferon responses, and cell survival under stress conditions. DTX3L’s E3 ligase activity contributes to ubiquitination of target proteins, influencing their stability or localization. Dysregulation of DTX3L has been linked to cancers, particularly B-cell lymphomas, and autoimmune diseases. Its dual role in ubiquitination and ADP-ribosylation pathways positions it as a potential therapeutic target. Ongoing research focuses on elucidating its substrate specificity and regulatory mechanisms in cellular homeostasis and disease contexts.
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